Search Results (4)

Background and Clinical Significance: Currently, maxillary sinus floor elevation is one of the most common procedures used in implantology practice. Despite its predictability, the technique is not without complications, such as graft material dispersion in the sinus cavity, wound dehiscence, hematoma, fenestrations, oroantral fistulas, epistaxis, acute sinusitis, and Schneider membrane perforations. The treatment of the latter can be complex, and depending on its extent, surgery deferral may be necessary, leading to increased patient morbidity. Case Presentation: A patient with apical surgery underwent sinus floor elevation with a significant Schneider membrane perforation using a new approach involving titanium mesh, resorbable membrane, and xenograft. This allowed the continuation of surgery, reducing the number of interventions and patient morbidity. Conclusions: Despite limitations due to a small sample size, this case report demonstrates that addressing large Schneider membrane perforations and placing implants is effective and predictable using the technology and approach of mesh formwork with titanium. Full article

The Posterior Superior Alveolar Artery (PSAA) provides vascular support to molars, gingiva, and maxillary sinus. A tear of the PSAA may cause profuse hemorrhages which may lead to complications at a surgical level. As such, it becomes crucial to anatomically analyse several features regarding the PSAA as well as the area surrounding it. In this paper, we are particularly interested in the study of the complexity of the periodontal tissue structure which appears close to the location of the PSAA. A total amount of 400 cone beam computed tomography (CBCT) scans (two per subject) were performed to explore the presence of the PSAA, the thickness of the Schneider’s membrane, and the existence of septa. Several parameters were evaluated including the location of the artery in the maxillary sinus, the distance from the PSAA to the alveolar ridge, the thickness of the membrane, the diameter of the cavities produced by the septa, and the fractal dimension of the trabecular tissue that surrounds the PSAA. They were found strong linear relationships between Distal and Central Measures (a Pearson’s $R^2=0.9952$ ), Mesial and Central Measures ( $R^2=0.9950$ ), and Distal and Mesial Measure ( $R^2=0.997$ ). We hypothesised that the loss of dental pieces would imply a distinct complexity of the trabecular tissue structure surrounding the PSAA. In this way, a p-value equal to $0.001$ was provided by the Mann-Whitney test, which supports our hypothesis. Furthermore, the mean of the fractal dimensions of the group of edentulous patients (equal to $1.56$ ) was found to be lower than the one of the group of non-edentulous patients (equal to $1.61$ ) with small standard deviations in both cases. Our study suggests that accurate calculations of the fractal dimension combined with the use of CBCT do provide valuable information regarding the area that surrounds the PSAA. Full article

Drosophila Schneider 2 (S2) cells are a simple and powerful system commonly used in cell biology because they are well suited for high resolution microscopy and RNAi-mediated depletion. However, understanding dynamic processes, such as cell division, also requires methodology to interfere with protein function with high spatiotemporal control. In this research study, we report the adaptation of an optogenetic tool to Drosophila S2 cells. Light-activated reversible inhibition by assembled trap (LARIAT) relies on the rapid light-dependent heterodimerization between cryptochrome 2 (CRY2) and cryptochrome-interacting bHLH 1 (CIB1) to form large protein clusters. An anti-green fluorescent protein (GFP) nanobody fused with CRY2 allows this method to quickly trap any GFP-tagged protein in these light-induced protein clusters. We evaluated clustering kinetics in response to light for different LARIAT modules, and showed the ability of GFP-LARIAT to inactivate the mitotic protein Mps1 and to disrupt the membrane localization of the polarity regulator Lethal Giant Larvae (Lgl). Moreover, we validated light-induced co-clustering assays to assess protein-protein interactions in S2 cells. In conclusion, GFP-based LARIAT is a versatile tool to answer different biological questions, since it enables probing of dynamic processes and protein-protein interactions with high spatiotemporal resolution in Drosophila S2 cells. Full article

Plasma membrane intrinsic proteins (PIPs) are a subfamily of aquaporin proteins located on plasma membranes where they facilitate the transport of water and small uncharged solutes. PIPs play an important role throughout plant development, and in response to abiotic stresses. Jojoba (Simmondsia chinensis (Link) Schneider), as a typical desert plant, tolerates drought, salinity and nutrient-poor soils. In this study, a PIP1 gene (ScPIP1) was cloned from jojoba and overexpressed in Arabidopsis thaliana. The expression of ScPIP1 at the transcriptional level was induced by polyethylene glycol (PEG) treatment. ScPIP1 overexpressed Arabidopsis plants exhibited higher germination rates, longer roots and higher survival rates compared to the wild-type plants under drought and salt stresses. The results of malonaldehyde (MDA), ion leakage (IL) and proline content measurements indicated that the improved drought and salt tolerance conferred by ScPIP1 was correlated with decreased membrane damage and improved osmotic adjustment. We assume that ScPIP1 may be applied to genetic engineering to improve plant tolerance based on the resistance effect in transgenic Arabidopsis overexpressing ScPIP1. Full article