Search Results (2)

Moso bamboo (Phyllostachys edulis), renowned for its rapid growth, is attributed to the dynamic changes in its apical meristem. The CLAVATA3/EMBRYO SURROUNDING REGION-RELATED (CLE) family genes are known to play crucial roles in regulating meristem and organ formation in model plants, but their functions in Moso bamboo remain unclear. Here, we conducted a genome-wide identification of the CLE gene family of Moso bamboo and investigated their gene structure, chromosomal localization, evolutionary relationships, and expression patterns. A total of 11 PheCLE genes were identified, all of which contained a conserved CLE peptide core functional motif (Motif 1) at their C-termini. Based on Arabidopsis classification criteria, these genes were predominantly distributed in Groups A–C. Collinearity analysis unveiled significant synteny among CLE genes in Moso bamboo, rice, and maize, implying potential functional conservation during monocot evolution. Transcriptomic analysis showed significant expression of these genes in the apical tissues of Moso bamboo, including root tips, shoot tips, rhizome buds, and flower buds. Particularly, single-cell transcriptomic data and in situ hybridization further corroborated the heightened expression of PheCLE1 and PheCLE10 in the apical tissue of basal roots. Additionally, the overexpression of PheCLE1 and PheCLE10 in rice markedly promoted root growth. PheCLE1 and PheCLE10 were both located on the cell membrane. Furthermore, the upstream transcription factors NAC9 and NAC6 exhibited binding affinity toward the promoters of PheCLE1 and PheCLE10, thereby facilitating their transcriptional activation. In summary, this study not only systematically identified the CLE gene family in Moso bamboo for the first time but also emphasized their central roles in apical tissue development. This provides a valuable theoretical foundation for the further exploration of functional peptides and their signaling regulatory networks in bamboo species. Full article

The phenyltetrel pyridine-2-olates PhE(pyO)₃ (E = Si, Ge, Sn; pyO = pyridine-2-olate) were synthesized from the respective chlorides PhECl₃ and 2-hydroxypyridine (2-pyridone) with the aid of a sacrificial base (triethylamine). Their solid-state structures were determined by single-crystal X-ray diffraction. PhSi(pyO)₃ exhibits a three-fold capped tetrahedral Si coordination sphere ([4+3]-coordination, Si···N separations ca. 3.0 Å), in accordance with structures of previously reported silicon pyridine-2-olates. PhGe(pyO)₃ adopts a related [4+3]-coordination mode, which differs in terms of the tetrahedral faces capped by the pyridine N atoms. Additionally, shorter Ge···N separations (2.8–2.9 Å) indicate a trend toward tetrel hypercoordination. PhSn(pyO)₃ features heptacoordinate tin within a pentagonal bipyramidal Sn coordination sphere (Sn···N separations 2.2–2.4 Å). For the Si and Sn compounds, ²⁹Si and ¹¹⁹Sn NMR spectroscopy indicates retention of their tetrel coordination number in chloroform solution. Full article