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Keywords = NASBA-assisted amplification

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15 pages, 1118 KB  
Article
An Isothermal Deoxyribozyme Sensor for Rapid Detection of Enteroviral RNA
by Begüm Şaş, Anastasiia Dmitrievna Kirichenko, Marina Anatolyevna Kapitonova, Anna Vyacheslavovna Shabalina, Olga Ilyinichna Kanaeva, Tamer Mohammed El-Messery, Vladimir Georgievich Dedkov and Anna Sergeevna Dolgova
Biosensors 2025, 15(9), 562; https://doi.org/10.3390/bios15090562 - 27 Aug 2025
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Abstract
Enteric viruses are a major cause of waterborne infections due to their high environmental stability and extremely low infectious dose. Current molecular diagnostic methods, while accurate, often depend on thermal cycling and centralized laboratory facilities, limiting their applicability in decentralized or resource-limited settings. [...] Read more.
Enteric viruses are a major cause of waterborne infections due to their high environmental stability and extremely low infectious dose. Current molecular diagnostic methods, while accurate, often depend on thermal cycling and centralized laboratory facilities, limiting their applicability in decentralized or resource-limited settings. In this study, we developed an isothermal biosensor based on a split deoxyribozyme that reconstitutes its catalytic core upon hybridization with a conserved sequence of enteroviral RNA. This activation leads to site-specific cleavage of a fluorogenic substrate, producing a quantifiable fluorescent signal. The system was experimentally validated using both synthetic enteroviral RNA and RNA extracted from environmental water samples. To enhance detection sensitivity, the DNAzyme-based assay was coupled with isothermal RNA amplification. The results demonstrate high selectivity and compatibility with real-world samples, supporting the sensor’s utility for field-deployable viral RNA detection. Overall, this study highlights the potential of the DNAzyme-based platform as a portable, sequence-specific, and amplification-assisted diagnostic tool for environmental surveillance of enteric viruses. Full article
(This article belongs to the Section Environmental Biosensors and Biosensing)
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