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The Maigaiti Slope is a significant oil-gas-bearing field in the Tarim Basin. Based on 3D and 2D seismic data, systematic interpretation, stage sorting and genetic analysis of strike-slip faults in the Maigaiti Slope were carried out for the first time. The relationship between strike-slip faults and hydrocarbon accumulation was studied in combination with the fine dissection of hydrocarbon reservoirs. The study suggested that: (1) Staging and segmentation characteristics of strike-slip faults are evidently presented in the Maigaiti Slope. According to active periods, strike-slip faults can be divided into early Caledonian period, late Caledonian period, Hercynian period, and Himalayan period. According to plane distribution characteristics, strike-slip faults can be divided into the west Maigaiti Segment, mid-Maigaiti Segment, Madong Segment, and Bachu Segment. The main active periods and plane distribution of strike-slip faults in different sections are remarkably different. This analysis suggests that it is the response to multi-period and multi-directional tectonic movements, which are primarily dominated by the migration and evolution of the Hetian paleo-uplifts. (2) The coupling relationship between the active period of strike-slip faults and the trap forming period is the key to hydrocarbon accumulation in the Carboniferous–Ordovician, which determines the petroleum properties and enrichment horizon of the Cambrian post-salt system; medium-heavy oil is in the Caledonian period, light oil in the Indosinian period, and dry gas in the Himalayan period. Full article