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Keywords = Limosilactobacillus fermentum phage LFP02

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13 pages, 1732 KiB  
Article
Characteristics and Whole-Genome Analysis of Limosilactobacillus fermentum Phage LFP02
by Ruirui Lv, Xin Gao, Can Zhang, Weiqi Lian, Xingyu Quan, She Guo and Xia Chen
Foods 2023, 12(14), 2716; https://doi.org/10.3390/foods12142716 - 16 Jul 2023
Cited by 4 | Viewed by 2197
Abstract
Limosilactobacillus fermentum is a bacterium widely used in food production, medicine, and industrial fermentation. However, fermentation could fail due to phage contamination. L. fermentum bacteriophage LFP02 can be induced from L. fermentum IMAU 32579 using mitomycin C. To better understand the characteristics of [...] Read more.
Limosilactobacillus fermentum is a bacterium widely used in food production, medicine, and industrial fermentation. However, fermentation could fail due to phage contamination. L. fermentum bacteriophage LFP02 can be induced from L. fermentum IMAU 32579 using mitomycin C. To better understand the characteristics of this phage, its physiological and genomic characteristics were evaluated. The results showed that its optimal multiplicity of infection was 0.01, and the burst size was 148.03 ± 2.65 pfu/infective center. Compared to temperature, pH had a more obvious influence on phage viability, although its adsorption capacity was not affected by the divalent cations (Ca2+ and Mg2+) or chloramphenicol. Its genome size was 43,789 bp and the GC content was 46.06%, including 53 functional proteins. Compared to other L. fermentum phages, phage LFP02 had chromosome deletion, insertion, and inversion, which demonstrated that it was a novel phage. This study could expand the knowledge of the biological characteristics of L. fermentum bacteriophages and provide some theoretical basis for bacteriophage prevention during fermentation. Full article
(This article belongs to the Section Foodomics)
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