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Keywords = LOVTRAP

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18 pages, 2001 KB  
Article
Plug-and-Display Photo-Switchable Systems on Plant Virus Nanoparticles
by Louisa Kauth, Eva Miriam Buhl, Julian Luka, Karolin Richter, Ulrich Commandeur and Christina Dickmeis
BioTech 2022, 11(4), 49; https://doi.org/10.3390/biotech11040049 - 21 Oct 2022
Cited by 1 | Viewed by 3582
Abstract
Light can be used to regulate protein interactions with a high degree of spatial and temporal precision. Photo-switchable systems therefore allow the development of controllable protein complexes that can influence various cellular and molecular processes. Here, we describe a plant virus-based nanoparticle shuttle [...] Read more.
Light can be used to regulate protein interactions with a high degree of spatial and temporal precision. Photo-switchable systems therefore allow the development of controllable protein complexes that can influence various cellular and molecular processes. Here, we describe a plant virus-based nanoparticle shuttle for the distribution of proteins that can be released when exposed to light. Potato virus X (PVX) is often used as a presentation system for heterologous proteins and epitopes, and has ideal properties for biomedical applications such as good tissue penetration and the ability to form hydrogels that present signaling molecules and promote cell adhesion. In this study, we describe three different systems attached to the surface of PVX particles: LOVTRAP, BphP1/QPAS1 and Dronpa145N. We demonstrated the functionality of all three photo-switchable protein complexes in vitro and the successful loading and unloading of PVX particles. The new systems provide the basis for promising applications in the biomedical and biomaterial sciences. Full article
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