Ten compounds, including three lignan glycosides and seven lignans, were purified from
Justicia procumbens L. in 8 h using an efficient strategy based on high-speed counter-current chromatography (HSCCC). The two-phase solvent system composed of petroleum–ethyl acetate–methanol–H
2O (1:0.7:1:0.7,
v/
v)
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Ten compounds, including three lignan glycosides and seven lignans, were purified from
Justicia procumbens L. in 8 h using an efficient strategy based on high-speed counter-current chromatography (HSCCC). The two-phase solvent system composed of petroleum–ethyl acetate–methanol–H
2O (1:0.7:1:0.7,
v/
v) was firstly employed to separate the crude extract (320 mg), from which 19.3 mg of justicidin B (
f), 10.8 mg of justicidin A (
g), 13.9 mg of 6′-hydroxyjusticidin C (
h), 7.7 mg of justicidin E (
i), 6.3 mg of lignan J
1 (
j) were obtained with 91.3 mg of enriched mixture of compounds
a–
e. The enriched mixture (91.3 mg) was further separated using the solvent system consisting of petroleum–ethyl acetate–methanol–H
2O (3:3.8:3:3.8,
v/
v), yielding 12.1 mg of procumbenoside E (
a); 7.6 mg of diphyllin-1-
O-β-
d-apiofuranoside (
b); 7.4 mg of diphyllin (
c); 8.3 mg of 6′-hydroxy justicidin B (
d); and 7.9 mg of diphyllin acetyl apioside (
e). The purities of the 10 components were all above 94%, and their structures were identified by NMR and ESI-MS spectra. The results demonstrated that the strategy based on HSCCC for the separation of lignans and their glycosides was efficient and rapid.
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