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Keywords = GhCAD6

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12 pages, 4294 KB  
Article
Overexpression of GhCAD6 in Upland Cotton (Gossypium hirsutum L.) Enhances Fiber Quality and Increases Lignin Content in Fibers
by Zumuremu Tuerxun, Chenyu Li, Xiaorong Li, Yuanxin Li, Xinxin Qin, Hui Zhang, Yang Yang, Guo Chen, Juan Li, Zhigang Liu, Xunji Chen, Darun Cai and Bo Li
Int. J. Mol. Sci. 2025, 26(19), 9518; https://doi.org/10.3390/ijms26199518 - 29 Sep 2025
Cited by 1 | Viewed by 481
Abstract
Cotton is a vital economic crop, and cotton fiber serves as the primary raw material for the textile industry. Lignin in cotton fiber is closely associated with fiber quality. Lignin is synthesized through the phenylpropanoid metabolic pathway, where the cinnamyl alcohol dehydrogenase gene [...] Read more.
Cotton is a vital economic crop, and cotton fiber serves as the primary raw material for the textile industry. Lignin in cotton fiber is closely associated with fiber quality. Lignin is synthesized through the phenylpropanoid metabolic pathway, where the cinnamyl alcohol dehydrogenase gene CAD6 plays a significant role. In this study, we obtained successfully transformed overexpression plants by constructing an overexpression vector and performing genetic transformation and tissue culture. To verify the function of the GhCAD6 gene in upland cotton, we analyzed the agronomic traits, fiber quality, cell wall structure, and lignin content of GhCAD6-overexpressing plants. Our results indicate that the GhCAD6 gene is predominantly expressed during the stages of fiber elongation and secondary wall synthesis. Overexpression of the GhCAD6 gene resulted in increased plant lignin content and fiber upper half mean length, boll number per plant, fiber uniformity index, strength, and lint were improved. The fiber surface was smoother, and the fiber cell wall was more compact. These findings demonstrate that the GhCAD6 gene positively regulates lignin synthesis and fiber quality formation, contributing to the enhancement of cotton fiber quality. Full article
(This article belongs to the Section Molecular Genetics and Genomics)
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12 pages, 2730 KB  
Article
Golden Hull: A Potential Biomarker for Assessing Seed Aging Tolerance in Rice
by Jing Ye, Chengjing Wang, Ling Chen, Rongrong Zhai, Mingming Wu, Yanting Lu, Faming Yu, Xiaoming Zhang, Guofu Zhu and Shenghai Ye
Agronomy 2024, 14(10), 2357; https://doi.org/10.3390/agronomy14102357 - 12 Oct 2024
Viewed by 1560
Abstract
Seed aging is a complex process that involves various physiological and biochemical changes leading to a decline in seed viability during storage. However, the specific biomarkers for assessing the degree of seed aging in rice remain elusive. In this study, we isolated a [...] Read more.
Seed aging is a complex process that involves various physiological and biochemical changes leading to a decline in seed viability during storage. However, the specific biomarkers for assessing the degree of seed aging in rice remain elusive. In this study, we isolated a golden hull mutant, gh15, from the indica rice Z15 by employing a radiation mutagenesis technique. Compared with the wild type (WT) Z15, the mutant gh15 displayed a golden hue in the hull, stem, and internodes, while no significant differences were observed in the key agronomic traits. A genetic analysis showed that the gh15 phenotype is regulated by a single recessive gene, which possibly encodes cinnamyl alcohol dehydrogenase OsCAD2. Significant differences of seed aging tolerance were observed between gh15 and WT after six months of natural storage and artificial aging treatment, with gh15 exhibiting a markedly lower aging tolerance compared to the WT. Haplotype assays indicated that the Hap2 of OsCAD2 was significantly associated with the dark coloration of the hull and lower seed aging tolerance. The molecular marker of OsCAD2 associated with seed color was explored in rice. These findings demonstrate that the golden hull serves as a potential biomarker for the rapid assessment of seed aging tolerance in rice. Full article
(This article belongs to the Special Issue Innovative Research on Rice Breeding and Genetics)
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