ATP synthase catalyzes the synthesis of ATP by consuming the proton electrochemical gradient, which is essential for maintaining the life activity of organisms. The peripheral stalk belongs to ATP synthase and plays an important supporting role in the structure of ATP synthase, but
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ATP synthase catalyzes the synthesis of ATP by consuming the proton electrochemical gradient, which is essential for maintaining the life activity of organisms. The peripheral stalk belongs to ATP synthase and plays an important supporting role in the structure of ATP synthase, but their regulation in filamentous fungi are not yet known. Here, we characterized the subunits of the peripheral stalk,
FfATPh,
FfATP5, and
FfATPb, and explored their functions on development and pathogenicity of
Fusarium Fujikuroi. The
FfATPh,
FfATP5, and
FfATPb deletion mutations (
∆FfATPh,
∆FfATP5, and
∆FfATPb) presented deficiencies in vegetative growth, sporulation, and pathogenicity. The sensitivity of
∆FfATPh,
∆FfATP5, and
∆FfATPb to fludioxonil, phenamacril, pyraclostrobine, and fluazinam decreased. In addition,
∆FfATPh exhibited decreased sensitivity to ionic stress and osmotic stress, and
∆FfATPb and
∆FfATP5 were more sensitive to oxidative stress.
FfATPh,
FfATP5, and
FfATPb were located on the mitochondria, and
∆FfATPh,
∆FfATPb, and
∆FfATP5 disrupted mitochondrial location. Furthermore, we demonstrated the interaction among FfATPh, FfATP5, and FfATPb by Bimolecular Fluorescent Complimentary (BiFC) analysis. In conclusion,
FfATPh,
FfATP5, and
FfATPb participated in regulating development, pathogenicity, and sensitivity to fungicides and stress factors in
F. fujikuroi.
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