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Keywords = Crlz-1

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Article
Crlz-1 Homozygous Null Knockout Mouse Embryos Are Lethally Stopped in Their Early Development
by Seung-Young Choi, Joo-Hyun Pi, So-Eun Jeong and Chang-Joong Kang
Genes 2022, 13(3), 511; https://doi.org/10.3390/genes13030511 - 14 Mar 2022
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Abstract
Although the conditional gene knockout (KO) is a better choice for observing its phenotype in a specific cell, tissue, and/or organ, the simple null gene KO could nevertheless be attempted initially to scan its overall phenotypes at the level of the whole-body system, [...] Read more.
Although the conditional gene knockout (KO) is a better choice for observing its phenotype in a specific cell, tissue, and/or organ, the simple null gene KO could nevertheless be attempted initially to scan its overall phenotypes at the level of the whole-body system, especially for a new gene such as Crlz-1. Therefore, with a hope to glean phenotypic clues for Crlz-1 at the whole-body system, we attempted to generate its null KO mice. Contrary to our original desire, Crlz-1 homozygous null KO mice were not born. However, in the chasing of their homozygous KO embryos, they were found to be lethally impaired from early development, remaining in a state of small globular mass without ever leading to a body shape, indicating the critical role of Crlz-1 as a Wnt target gene for the proliferation and/or differentiation of cells during early mouse embryonic development. Full article
(This article belongs to the Section Animal Genetics and Genomics)
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