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Keywords = Agrobacterium tumefasciens

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11 pages, 830 KiB  
Article
Lignin-Degrading Bacteria in Paper Mill Sludge
by Magda Rodriguez-Yupanqui, Magaly De La Cruz-Noriega, Claudio Quiñones, Nélida Milly Otiniano, Medardo A. Quezada-Alvarez, Walter Rojas-Villacorta, Gino A. Vergara-Medina, Frank R. León-Vargas, Haniel Solís-Muñoz and Segundo Rojas-Flores
Microorganisms 2023, 11(5), 1168; https://doi.org/10.3390/microorganisms11051168 - 29 Apr 2023
Cited by 4 | Viewed by 3369
Abstract
The effluents generated in the paper industry, such as black liquor, have a high content of lignin and other toxic components; however, they represent a source of lignin-degrading bacteria with biotechnological potential. Therefore, the present study aimed to isolate and identify lignin-degrading bacteria [...] Read more.
The effluents generated in the paper industry, such as black liquor, have a high content of lignin and other toxic components; however, they represent a source of lignin-degrading bacteria with biotechnological potential. Therefore, the present study aimed to isolate and identify lignin-degrading bacteria species in paper mill sludge. A primary isolation was carried out from samples of sludge present in environments around a paper company located in the province of Ascope (Peru). Bacteria selection was made by the degradation of Lignin Kraft as the only carbon source in a solid medium. Finally, the laccase activity (Um-L−1) of each selected bacteria was determined by oxidation of 2,2′-azinobis-(3-etilbencenotiazolina-6-sulfonate) (ABTS). Bacterial species with laccase activity were identified by molecular biology techniques. Seven species of bacteria with laccase activity and the ability to degrade lignin were identified. The bacteria Agrobacterium tumefasciens (2), Klebsiella grimontii (1), and Beijeinckia fluminensis (1) were reported for first time. K. grimowntii and B. fluminensis presented the highest laccase activity, with values of 0.319 ± 0.005 UmL−1 and 0.329 ± 0.004 UmL−1, respectively. In conclusion, paper mill sludge may represent a source of lignin-degrading bacteria with laccase activity, and they could have potential biotechnological applications. Full article
(This article belongs to the Section Environmental Microbiology)
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