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Keywords = ATP-dependent DNA helicase (TaDHL)

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10 pages, 2213 KiB  
Article
ATP-dependent DNA helicase (TaDHL), a Novel Reduced-Height (Rht) Gene in Wheat
by Baojin Guo, Xuemei Jin, Jingchuan Chen, Huiyan Xu, Mingxia Zhang, Xing Lu, Rugang Wu, Yan Zhao, Ying Guo, Yanrong An and Sishen Li
Genes 2022, 13(6), 979; https://doi.org/10.3390/genes13060979 - 30 May 2022
Cited by 11 | Viewed by 4368
Abstract
In wheat, a series of dwarf and semi-dwarf plant varieties have been developed and utilized worldwide since the 1960s and caused the ‘Green Revolution’. To date, 25 reduced-height (Rht) genes have been identified, but only several genes for plant height (PH) [...] Read more.
In wheat, a series of dwarf and semi-dwarf plant varieties have been developed and utilized worldwide since the 1960s and caused the ‘Green Revolution’. To date, 25 reduced-height (Rht) genes have been identified, but only several genes for plant height (PH) have been isolated previously. In this study, we identified a candidate gene, ATP-dependent DNA helicase (TaDHL-7B), for PH via QTL mapping and genome-wide association study (GWAS) methods. We knocked out this gene using the CRISPR/Cas9 system in variety ‘Fielder’. Two homozygous mutant genotypes, AAbbDD (−5 bp) and AAbbDD (−1 bp), were obtained in the T2 generation. The PH values of AAbbDD (−5 bp) and AAbbDD (−1 bp) were significantly reduced compared with the wild-type (WT, ‘Fielder’), indicating that TaDHL-7B is a novel Rht gene that controls the PH. This is the first time that a PH gene of wheat has been isolated with a non-hormone pathway, providing a new insight into the genetic control of PH. The TaDHL gene reduced the PH without a yield penalty. It could be used to improve the lodging resistance and yield in wheat breeding programs. Full article
(This article belongs to the Special Issue Genetics Studies on Wheat)
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