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Keywords = 3D microtrap chip

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18 pages, 5270 KB  
Article
Hemagglutination Assay via Optical Density Characterization in 3D Microtrap Chips
by Sung-Wook Nam, Dong-Gyu Jeon, Young-Ran Yoon, Gang Ho Lee, Yongmin Chang and Dong Il Won
Biosensors 2023, 13(7), 733; https://doi.org/10.3390/bios13070733 - 14 Jul 2023
Cited by 8 | Viewed by 4480
Abstract
Hemagglutination assay has been used for blood typing and detecting viruses, thus applicable for the diagnosis of infectious diseases, including COVID-19. Therefore, the development of microfluidic devices for fast detection of hemagglutination is on-demand for point-of-care diagnosis. Here, we present a way to [...] Read more.
Hemagglutination assay has been used for blood typing and detecting viruses, thus applicable for the diagnosis of infectious diseases, including COVID-19. Therefore, the development of microfluidic devices for fast detection of hemagglutination is on-demand for point-of-care diagnosis. Here, we present a way to detect hemagglutination in 3D microfluidic devices via optical absorbance (optical density, OD) characterization. 3D printing is a powerful way to build microfluidic structures for diagnostic devices. However, mixing liquid in microfluidic chips is difficult due to laminar flow, which hampers practical applications such as antigen-antibody mixing. To overcome the issue, we fabricated 3D microfluidic chips with embedded microchannel and microwell structures to induce hemagglutination between red blood cells (RBCs) and antibodies. We named it a 3D microtrap chip. We also established an automated measurement system which is an integral part of diagnostic devices. To do this, we developed a novel way to identify RBC agglutination and non-agglutination via the OD difference. By adapting a 3D-printed aperture to the microtrap chip, we obtained a pure absorbance signal from the microchannels by eliminating the background brightness of the microtrap chip. By investigating the underlying optical physics, we provide a 3D device platform for detecting hemagglutination. Full article
(This article belongs to the Topic Advances in Microfluidics and Lab on a Chip Technology)
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