Variation in plant species and extraction solvents play a crucial role in the recovery of their bioactive compounds and antifungal efficacy. Thus, in this study, a comparative investigation was carried out using extraction solvents: 70% acetone and 95% ethanol to obtain crude aqueous
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Variation in plant species and extraction solvents play a crucial role in the recovery of their bioactive compounds and antifungal efficacy. Thus, in this study, a comparative investigation was carried out using extraction solvents: 70% acetone and 95% ethanol to obtain crude aqueous extracts from
Helichrysum odoratissimum and
H. patulum. Crude aqueous extracts were screened using gas chromatography–mass spectrometry (GC–MS), to gain insight into their chemical composition. Phytochemical properties (total polyphenols (TP) and radical scavenging capacity via 2,2-diphenyl-1-picrylhydrazyl (DPPH)), and antifungal activity against
Botrytis cinerea of the crude extracts were evaluated. Fungicide (Rovral
® WP) and extraction solvents were used as controls. Variation in
Helichrysum spp. and extraction solvent had influence on the chemical composition, phytochemicals, and antifungal activities. Metabolites such as γ-terpinene (≈0.1%), α-amorphene (≈0.6%) α-gurjunene (≈1.4%), β-selinene (2.2–3.2%), γ-gurjunene (≈3.3%), and methyl cinnamate (≈20%) were detected only in extracts of
H. patulum. Crude extract of
H. odoratissimum using 70% acetone had the highest TP (19.3 ± 0.76 g GA 100 g
−1), and DPPH capacity (13,251.5 ± 700.55 µmol Trolox g
−1) compared to
H. patulum (
p ≤ 0.05). Ethanolic extracts of
H. patulum showed highest antifungal efficacy (≈65%) against
B. cinerea (
p ≤ 0.05) compared to other crude extracts. This study showed that
Helichrysum spp. differ in their potential as a source for bioactive compounds and antifungal treatments/formulations.
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