Search Results (2)

Dendrocalamus brandisii is a high-quality bamboo species that can be used for both bamboo shoots and wood. The nutritional components and flavors of D. brandisii vary from different geographical provenances. However, the unique biological characteristics of bamboo make morphological classification methods unsuitable for distinguishing them. Although the new cultivar ‘Manxie No.1’ has significant differences in the branch characteristics and the color of shoot sheaths compared to the D. brandisii, it still lacks precise genetic information at the molecular level. This study identified 231,789 microsatellite markers based on the whole genome of D. brandisii and analyzed their type composition and distribution on chromosomes in detail. Then, using TP-M13-SSR fluorescence-labeling technology, 34 pairs of polymorphic primers were screened to identify the new cultivar ‘Manxie No.1’ and 11 different geographical provenances of D. brandisii. We also constructed DNA fingerprinting profiles for them. At the same time, we mapped six polymorphic SSRs to the gene of D. brandisii, among which SSR673 was mapped to DhB10G011540, which is related to plant immunity. The specific markers selected in this study can rapidly identify the provenances and the new cultivar of D. brandisii and help explore candidate genes related to some important traits. Full article

Cambrian subsalt dolomite is an important strategic area for natural gas exploration in the Tarim Basin. The gypsum-salt rocks, argillaceous mudstone and argillaceous dolomite strata developed in large areas of the Middle Cambrian can be used as good caprocks. The sealing ability and favorable area distribution of the Middle Cambrian caprock in the Tarim Basin are studied through the lithofacies paleogeography and microscopic evaluation of the Middle Cambrian strata in this paper. Based on the 2D seismic interpretation covering the entire basin, combined with data from drilling, outcrops, well logging, core samples and thin sections, the sedimentary characteristics and lithofacies paleogeography of the Middle Cambrian were studied and then the thickness of the Middle Cambrian gypsum-salt rocks, gypsiferous mudstone and gypsiferous dolomite was analyzed in the Tarim Basin. Studies suggest that the Middle Cambrian is primarily characterized by the development of restricted-platform facies. In the Awati Depression, the northern part of the Tazhong Uplift, the southern part of the Manxi Low Uplift, and the central and northern parts of the Bachu Uplift, the thickness of the gypsum-salt rock strata is relatively large. Moreover, centered on the northern part of the Bachu Uplift, the thickness of the gypsum-salt rocks decreases irregularly towards the periphery, forming a circumferential distribution. To investigate the sealing ability of caprocks, 64 core samples from four wells were examined under a microscope, and physical parameters as well as breakthrough-pressure tests were conducted. By establishing correlations between various parameters, the sealing ability of different rock types in the Cambrian formation within the study area was quantitatively assessed. The research suggests that gypsum-salt rocks exhibit superior sealing ability compared to gypsiferous mudstone and gypsiferous dolomite, but factors such as faults and geological conditions of gypsum can influence the sealing performance of caprocks. According to both micro- and macro-scale evaluations of the Cambrian strata in the study area, along with constraints imposed by actual drilling exploration results, a comprehensive evaluation method for assessing caprock sealing ability has been established. The results suggest that the Awat Depression, the western and southern parts of the Manxi Low Uplift, the northern and western parts of the Tazhong Uplift, and the central part of the Tabei Uplift are favorable areas for the development of caprocks. Full article