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Authors = Teruhiko Nitoda

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12 pages, 208 KiB  
Article
Effects of Quercetin on Mushroom Tyrosinase and B16-F10 Melanoma Cells
by Isao Kubo, Teruhiko Nitoda and Ken-ichi Nihei
Molecules 2007, 12(5), 1045-1056; https://doi.org/10.3390/12051045 - 15 May 2007
Cited by 59 | Viewed by 23726
Abstract
In searching for tyrosinase inhibitors from plants using L-3,4-dihydroxyphenylalanine (L-DOPA) as a substrate, quercetin was found to be partially oxidized to the corresponding o-quinone under catalysis by mushroom tyrosinase (EC 1.14.18.1). Simultaneously, L-DOPA was also oxidized to dopaquinone and [...] Read more.
In searching for tyrosinase inhibitors from plants using L-3,4-dihydroxyphenylalanine (L-DOPA) as a substrate, quercetin was found to be partially oxidized to the corresponding o-quinone under catalysis by mushroom tyrosinase (EC 1.14.18.1). Simultaneously, L-DOPA was also oxidized to dopaquinone and both o-quinones were further oxidized, respectively. The remaining quercetin partially formed adducts with dopaquinone through a Michael type addition. In general, flavonols form adducts with dopaquinone as long as their 3-hydroxyl group is free. Quercetin enhanced melanin production per cell in cultured murine B16-F10 melanoma cells, but this effect may be due in part to melanocytotoxicity. The concentration leading to 50% viable cells lost was established as 20 μM and almost complete lethality was observed at 80 μM. Full article
(This article belongs to the Special Issue Phenolics and Polyphenolics)
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