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Authors = Pawel Wielgus

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10 pages, 634 KiB  
Article
Visualization of the Differential Transition State Stabilization within the Active Site Environment
by Pawel Kedzierski, Pawel Wielgus, Adrian Sikora, W. Andrzej Sokalski and Jerzy Leszczynski
Int. J. Mol. Sci. 2004, 5(4), 186-195; https://doi.org/10.3390/i5040186 - 31 May 2004
Cited by 5 | Viewed by 10175
Abstract
Increasing interest in the enzymatic reaction mechanisms and in the nature of catalytic effects in enzymes causes the need of appropriate visualization methods. A new interactive method to investigate catalytic effects using differential transition state stabilization approach (DTSS) [1, 2] is presented. The [...] Read more.
Increasing interest in the enzymatic reaction mechanisms and in the nature of catalytic effects in enzymes causes the need of appropriate visualization methods. A new interactive method to investigate catalytic effects using differential transition state stabilization approach (DTSS) [1, 2] is presented. The catalytic properties of the active site of cytidine deaminase (E.C. 3.5.4.5) is visualized in the form of differential electrostatic properties. The visualization was implemented using scripting interface of VMD [3]. Cumulative Atomic Multipole Moments (CAMM) [4,5,6] were utilized for efficient yet accurate evaluation of the electrostatic properties. The implementation is efficient enough for interactive presentation of catalytic effects in the active site of the enzyme due to transition state or substrate movement. This system of visualization of DTTS approach can be potentially used to validate hypotheses regarding the catalytic mechanism or to study binding properties of transition state analogues. Full article
(This article belongs to the Special Issue Proceedings of the Workshop on Modeling Interaction in Biomolecules)
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