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Authors = Jose C. Orellana Palacios

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13 pages, 1618 KiB  
Article
Extraction Optimization, Functional and Thermal Properties of Protein from Cherimoya Seed as an Unexploited By-Product
by Jose C. Orellana-Palacios, Milad Hadidi, Marwa Yassamine Boudechiche, Maria Lopez S. Ortega, Diego J. Gonzalez-Serrano, Andres Moreno, Przemysław Łukasz Kowalczewski, Matteo Bordiga and Amin Mousavi Khanegah
Foods 2022, 11(22), 3694; https://doi.org/10.3390/foods11223694 - 18 Nov 2022
Cited by 23 | Viewed by 3225
Abstract
Plant-based proteins are gaining in attraction compared with animal-based proteins due to their superior ethical profiles, growing concerns on the part of various organizations about animal health and welfare, and increased global greenhouse-gas emissions in meat production. In this study, the response surface [...] Read more.
Plant-based proteins are gaining in attraction compared with animal-based proteins due to their superior ethical profiles, growing concerns on the part of various organizations about animal health and welfare, and increased global greenhouse-gas emissions in meat production. In this study, the response surface methodology (RSM) using a Box–Behnken design (BBD) was applied to optimize the ultrasound-assisted alkaline extraction of cherimoya-seed proteins as valuable by-products. The effects of three pH, temperature, and time factors on the protein-extraction yield and protein content were investigated. The pH at 10.5 and temperature of 41.8 °C for 26.1 min were considered the optimal ultrasound-assisted alkaline-extraction conditions since they provided the maximum extraction yield (17.3%) and protein content (65.6%). An established extraction technique was employed to enhance the cherimoya-seed protein yield, purity, and functional properties. A thermogravimetric analysis (TGA) of the samples showed that the ultrasound-assisted alkaline extraction improved the thermal stability of the protein concentrate. Full article
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33 pages, 9995 KiB  
Review
Spectrophotometric Methods for Measurement of Antioxidant Activity in Food and Pharmaceuticals
by Marios C. Christodoulou, Jose C. Orellana Palacios, Golnaz Hesami, Shima Jafarzadeh, José M. Lorenzo, Rubén Domínguez, Andres Moreno and Milad Hadidi
Antioxidants 2022, 11(11), 2213; https://doi.org/10.3390/antiox11112213 - 8 Nov 2022
Cited by 150 | Viewed by 31022
Abstract
In recent years, there has been a growing interest in the application of antioxidants in food and pharmaceuticals due to their association with beneficial health effects against numerous oxidative-related human diseases. The antioxidant potential can be measured by various assays with specific mechanisms [...] Read more.
In recent years, there has been a growing interest in the application of antioxidants in food and pharmaceuticals due to their association with beneficial health effects against numerous oxidative-related human diseases. The antioxidant potential can be measured by various assays with specific mechanisms of action, including hydrogen atom transfer, single electron transfer, and targeted scavenging activities. Understanding the chemistry of mechanisms, advantages, and limitations of the methods is critical for the proper selection of techniques for the valid assessment of antioxidant activity in specific samples or conditions. There are various analytical techniques available for determining the antioxidant activity of biological samples, including food and plant extracts. The different methods are categorized into three main groups, such as spectrometry, chromatography, and electrochemistry techniques. Among these assays, spectrophotometric methods are considered the most common analytical technique for the determination of the antioxidant potential due to their sensitivity, rapidness, low cost, and reproducibility. This review covers the mechanism of actions and color changes that occur in each method. Furthermore, the advantages and limitations of spectrophotometric methods are described and discussed in this review. Full article
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14 pages, 3419 KiB  
Article
Phenolics from Defatted Black Cumin Seeds (Nigella sativa L.): Ultrasound-Assisted Extraction Optimization, Comparison, and Antioxidant Activity
by Abdelkrim Gueffai, Diego J. Gonzalez-Serrano, Marios C. Christodoulou, Jose C. Orellana-Palacios, Maria Lopez S. Ortega, Aoumria Ouldmoumna, Fatima Zohra Kiari, Georgia D. Ioannou, Constantina P. Kapnissi-Christodoulou, Andres Moreno and Milad Hadidi
Biomolecules 2022, 12(9), 1311; https://doi.org/10.3390/biom12091311 - 16 Sep 2022
Cited by 30 | Viewed by 4774
Abstract
An ultrasound-assisted method was used for the extraction of phenolics from defatted black cumin seeds (Nigella sativa L.), and the effects of several extraction factors on the total phenolic content and DPPH radical scavenging activity were investigated. To improve the extraction efficiency [...] Read more.
An ultrasound-assisted method was used for the extraction of phenolics from defatted black cumin seeds (Nigella sativa L.), and the effects of several extraction factors on the total phenolic content and DPPH radical scavenging activity were investigated. To improve the extraction efficiency of phenolics from black cumin seed by ultrasonic-assisted extraction, the optimal extraction conditions were determined as follows: ethanol concentration of 59.1%, extraction temperature of 44.6 °C and extraction time of 32.5 min. Under these conditions, the total phenolic content and DPPH radical scavenging activity increased by about 70% and 38%, respectively, compared with conventional extraction. Furthermore, a complementary quantitative analysis of individual phenolic compounds was carried out using the HPLC-UV technique. The phenolic composition revealed high amounts of epicatechin (1.88–2.37 mg/g) and rutin (0.96–1.21 mg/g) in the black cumin seed extracts. Ultrasonic-assisted extraction can be a useful extraction method for the recovery of polyphenols from defatted black cumin seeds. Full article
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13 pages, 1567 KiB  
Review
Table Olive Wastewater as a Potential Source of Biophenols for Valorization: A Mini Review
by Alberto J. Huertas-Alonso, Diego J. Gonzalez-Serrano, Milad Hadidi, Manuel Salgado-Ramos, Jose C. Orellana-Palacios, M. Prado Sánchez-Verdú, Qiang Xia, Mario J. Simirgiotis, Francisco J. Barba, Basharat Nabi Dar and Andres Moreno
Fermentation 2022, 8(5), 215; https://doi.org/10.3390/fermentation8050215 - 9 May 2022
Cited by 8 | Viewed by 3726
Abstract
The table olive industry generates high amounts of wastewater annually during the alkaline treatment, fermentation, and washing steps of olives. High conductivity and salt content, as well as the high organic and biophenol contents of these waters, is a worldwide problem, especially in [...] Read more.
The table olive industry generates high amounts of wastewater annually during the alkaline treatment, fermentation, and washing steps of olives. High conductivity and salt content, as well as the high organic and biophenol contents of these waters, is a worldwide problem, especially in the Mediterranean region, which is the major table olive producing area. There is a wide variety of bioactives found in wastewater derived from table olive processing. The main compounds of table olive wastewater, such as those derived from phenolic, hydrocarbon, and sugar fractions, can be recovered and reused. In this review, the table olive manufacturing processes and the volumes and composition of wastewater generated from the different methods of table olive processing are discussed. In addition, biophenols of table olive water and their biological activities are also introduced. The high concentrations of valuable biophenols, such as tyrosol and hydroxytyrosol, show promising potential for valorizing table olive wastewater; however, more research is needed in this area. Full article
(This article belongs to the Special Issue Valorization of Waste from Agro-Food, Food and Marine Industry)
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