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The evaluation of geosites is an essential part of conserving the geodiversity and biodiversity of an ecosystem, as well as safeguarding the cultural, geological, environmental, and landscape wealth that a highly recognized geographical area possesses. In this context, Guayaquil, the pearl of the Pacific, is a city that registers, in its history and evolution, a binding relationship with the geo-biodiversity of the geosites that characterize it. This work aims to assess places of tourist interest in Guayaquil and its surrounding areas through a geo-environmental evaluation matrix to establish a sustainability proposal that promotes the geotourism development of the city. The methodology consisted of: (i) geographic registration of the chosen sites and present characteristics, (ii) environmental analysis of the sites based on the cause–effect method, and (iii) strategies for the inclusion of these sites into the geo-environmental and geotourism system of Guayaquil as potential geosites. The results demonstrate that Guayaquil has impressive geodiversity in several potential natural sites, obtaining highly representative values that reinforce the city’s natural diversity elements. Sites such as Cerro San Pedro and the Cerro San Eduardo, Pascuales, and Zeolites quarries have the most significant adverse environmental impacts. While places such as Cerro Azul, Estero Salado, Isla Santay, and Hornos de Cal (Bosque Protector Cerro Blanco), obtained positive values that highlight their environmental value, being of great benefit to the city and to nature. Furthermore, some of these places could be integrated into tourism development plans, and as potential geosites, they could complement various services and opportunities for discovering nature. Finally, all this can lead to a sustainable proposal for a Global Geopark project in Guayaquil based on the results obtained in this work. Full article