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Low-Field, Benchtop NMR Spectroscopy as a Potential Tool for Point-of-Care Diagnostics of Metabolic Conditions: Validation, Protocols and Computational Models

1
Leicester School of Pharmacy, De Montfort University, The Gateway, Leicester LE1 9BH, UK
2
Department of Chemical Sciences, University of Huddersfield, Queensgate, Huddersfield HD1 3DH, UK
3
Greater Manchester NHS Trust, Stepping Hill Hospital, Poplar Grove, Hazel Grove, Stockport SK2 7JE, UK
4
Magritek GmbH, Philipsstraße 8, 52068 Aachen, Germany
5
Department of Electrical and Electronic Engineering, University of Nottingham, University Park, Nottingham NG7 2RD, UK
6
Department of Chemistry, University of Loughborough, Epinal Way, Loughborough LE11 3TU, UK
*
Author to whom correspondence should be addressed.
High-Throughput 2019, 8(1), 2; https://doi.org/10.3390/ht8010002
Received: 4 October 2018 / Revised: 13 December 2018 / Accepted: 13 December 2018 / Published: 27 December 2018
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Abstract

Novel sensing technologies for liquid biopsies offer promising prospects for the early detection of metabolic conditions through omics techniques. Indeed, high-field nuclear magnetic resonance (NMR) facilities are routinely used for metabolomics investigations on a range of biofluids in order to rapidly recognise unusual metabolic patterns in patients suffering from a range of diseases. However, these techniques are restricted by the prohibitively large size and cost of such facilities, suggesting a possible role for smaller, low-field NMR instruments in biofluid analysis. Herein we describe selected biomolecule validation on a low-field benchtop NMR spectrometer (60 MHz), and present an associated protocol for the analysis of biofluids on compact NMR instruments. We successfully detect common markers of diabetic control at low-to-medium concentrations through optimised experiments, including α-glucose (≤2.8 mmol/L) and acetone (25 µmol/L), and additionally in readily accessible biofluids, particularly human urine. We present a combined protocol for the analysis of these biofluids with low-field NMR spectrometers for metabolomics applications, and offer a perspective on the future of this technique appealing to ‘point-of-care’ applications. View Full-Text
Keywords: metabolomics; benchtop 60 MHz NMR analysis; biomarkers; biomolecules; validation; protocol; diabetes metabolomics; benchtop 60 MHz NMR analysis; biomarkers; biomolecules; validation; protocol; diabetes
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Percival, B.C.; Grootveld, M.; Gibson, M.; Osman, Y.; Molinari, M.; Jafari, F.; Sahota, T.; Martin, M.; Casanova, F.; Mather, M.L.; Edgar, M.; Masania, J.; Wilson, P.B. Low-Field, Benchtop NMR Spectroscopy as a Potential Tool for Point-of-Care Diagnostics of Metabolic Conditions: Validation, Protocols and Computational Models. High-Throughput 2019, 8, 2.

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