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Volume 6
Issue 4
10.11599/germs.2016.1096
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Article

Frequency of Mutations in Rifampicin and Isoniazid Resistant Isolates of M. tuberculosis: An Analysis from Central India

by
Prabha Desikan
1,*,
Atul Kharate
2,
Nikita Panwalkar
3,
Jyoti Khurana
2,
Shaina Beg Mirza
3,
Aparna Chaturvedi
3,
Reeta Varathe
3,
Manju Chourey
3,
Pradeep Kumar
3,
Nitin Doshi
2 and
Manoj Pandey
4
1
National Reference Laboratory, Department of Microbiology, Bhopal Memorial Hospital and Research Center, Raisen Bypass Road, Karond, Bhopal, Madhya Pradesh, India
2
Intermediate Reference Laboratory, MRTB Hospital & Chest Center, Indore, Madhya Pradesh, India
3
National Reference Laboratory, Bhopal Memorial Hospital and Research Center, Bhopal, Madhya Pradesh, India
4
Department of Oncology, Bhopal Memorial Hospital and Research Center, Bhopal, Madhya Pradesh, India
*
Author to whom correspondence should be addressed.
GERMS 2016, 6(4), 125-131; https://doi.org/10.11599/germs.2016.1096
Submission received: 13 April 2016 / Revised: 26 July 2016 / Accepted: 23 August 2016 / Published: 2 December 2016
Versions Notes

Abstract

Background: The spread of drug-resistant tuberculosis has challenged tuberculosis control strategies globally. The present study aims to analyzes the frequency of mutations in rpoB, katG and inhA genes in strains of M. tuberculosis complex (MTBC) circulating in Central India. It is anticipated that the findings may provide a starting point to understand the evolutionary success of drug-resistant strains of MTBC in this region. Methods: Line probe assay was carried out on 720 consecutive sputum samples of MDR suspects from June 2012 to May 2013. Mutation frequencies in the rpoB, katG and inhA genes were analyzed. Results: Mutations were identified in 269 (37.6%) samples, as follows: 55 (7.6%) samples had mutations conferring resistance to only isoniazid, 84 (11.6%) had mutations conferring resistance to only rifampicin and 130 (18%) isolates had mutations conferring resistance to both isoniazid and rifampicin. The most frequent mutation in the rpoB gene was at codon S531L, seen in 141 (19.5%) isolates. The most frequent mutation in the katG gene was at codon S315T1, seen in 151 (20.9%) isolates; and in the inhA gene at codon C15T, seen in 21 (2.9%) isolates. Some unidentified mutations were also observed. Conclusion: The patterns and the frequency of the mutations identified in this study indicate the most frequent mutations at S531L codon in the rpoB gene, S315T1 codon in the katG gene and C15T codon in the promoter region of the inhA gene. Controlling the emergence and spread of MDR TB requires an understanding of the evolution of these mutations.
Keywords: mutation; frequency; Central India; M. tuberculosis mutation; frequency; Central India; M. tuberculosis

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MDPI and ACS Style

Desikan, P.; Kharate, A.; Panwalkar, N.; Khurana, J.; Mirza, S.B.; Chaturvedi, A.; Varathe, R.; Chourey, M.; Kumar, P.; Doshi, N.; et al. Frequency of Mutations in Rifampicin and Isoniazid Resistant Isolates of M. tuberculosis: An Analysis from Central India. GERMS 2016, 6, 125-131. https://doi.org/10.11599/germs.2016.1096

AMA Style

Desikan P, Kharate A, Panwalkar N, Khurana J, Mirza SB, Chaturvedi A, Varathe R, Chourey M, Kumar P, Doshi N, et al. Frequency of Mutations in Rifampicin and Isoniazid Resistant Isolates of M. tuberculosis: An Analysis from Central India. GERMS. 2016; 6(4):125-131. https://doi.org/10.11599/germs.2016.1096

Chicago/Turabian Style

Desikan, Prabha, Atul Kharate, Nikita Panwalkar, Jyoti Khurana, Shaina Beg Mirza, Aparna Chaturvedi, Reeta Varathe, Manju Chourey, Pradeep Kumar, Nitin Doshi, and et al. 2016. "Frequency of Mutations in Rifampicin and Isoniazid Resistant Isolates of M. tuberculosis: An Analysis from Central India" GERMS 6, no. 4: 125-131. https://doi.org/10.11599/germs.2016.1096

APA Style

Desikan, P., Kharate, A., Panwalkar, N., Khurana, J., Mirza, S. B., Chaturvedi, A., Varathe, R., Chourey, M., Kumar, P., Doshi, N., & Pandey, M. (2016). Frequency of Mutations in Rifampicin and Isoniazid Resistant Isolates of M. tuberculosis: An Analysis from Central India. GERMS, 6(4), 125-131. https://doi.org/10.11599/germs.2016.1096

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