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GERMS is published by MDPI from Volume 25 Issue 4 (2025). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with the former publisher Infection Science Forum S.R.L..
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Article

Experimental Approach for Bacteriophage Susceptibility Testing of Planktonic and Sessile Bacterial Populations—Study Protocol

by
Alina Cristina Neguț
1,*,
Oana Săndulescu
2,
Marcela Popa
3,4,
Anca Streinu-Cercel
2,
Zemphira Alavidze
5,
Ioana Berciu
2,
Coralia Bleotu
3,6,
Mircea Ioan Popa
7,
Mariana Carmen Chifiriuc
3,4 and
Adrian Streinu-Cercel
2
1
Carol Davila University of Medicine and Pharmacy, National Institute for Infectious Diseases “Prof. Dr. Matei Balş”, Bucharest, Romania
2
Department of Infectious Diseases, Carol Davila University of Medicine and Pharmacy, National Institute for Infectious Diseases “Prof. Dr. Matei Balş”, Bucharest, Romania
3
Microbiology Department, Faculty of Biology, University of Bucharest, Bucharest, Romania
4
Research Institute of the University of Bucharest, Bucharest, Romania
5
Phage Therapy Center, Tbilisi, Georgia
6
Ștefan S. Nicolau Institute of Virology, Romanian Academy, Bucharest, Romania
7
Department of Microbiology II, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania
*
Author to whom correspondence should be addressed.
GERMS 2014, 4(4), 92-96; https://doi.org/10.11599/germs.2014.1062
Submission received: 6 November 2014 / Revised: 27 November 2014 / Accepted: 28 November 2014 / Published: 1 December 2014
Versions Notes

Abstract

Introduction: Antimicrobial resistance is a growing threat for all clinical branches. This phenomenon poses important challenges in controlling infectious diseases. However, multidrug resistance is not the only issue, as bacteria that are otherwise susceptible to common antibiotics express other patterns for evading antibiotherapy, for example they can aggregate within a self-produced matrix to form biofilm. Methods: We intend to perform a prospective laboratory study of the germs isolated from different samples collected from patients admitted with infectious pathology in reference hospitals in Romania. We will perform antibiotic resistance testing as well as phage testing, both on solid and liquid growth medium, for Staphylococcus spp., Enterococcus spp., and Pseudomonas spp. We intend to collect data for 150 patients with different infections with these identified pathogens. Phage susceptibility testing will be performed using 5 types of strain-specific bacteriophage mixtures: PYO, INTESTI, STAPHYLOCOCCAL (Eliava BioPreparations, Tbilisi, Georgia), PHAGYO, PHAGESTI (JSC “Biochimpharm”, Tbilisi, Georgia). For phage-susceptible strains, we will evaluate biofilm formation in the presence of phages, as well as phage effect on already formed biofilm. Expected results: Through this study, we intend to provide the first set of results on bacteriophage-susceptibility of bacteria isolated from patients with hard to treat infections, from reference hospitals in Romania. By evaluating a large number of bacterial strains we aim to predict and project biofilm kinetics, while adding binary phage dilutions at key timepoints during biofilm formation. POSDRU/159/1.5/S/141531; Carol Davila University of Medicine and Pharmacy, Young Researchers Grant no. 28341/2013.
Keywords: bacteriophages; planktonic bacteria; biofilm; lysis; spectrophotometry bacteriophages; planktonic bacteria; biofilm; lysis; spectrophotometry

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MDPI and ACS Style

Neguț, A.C.; Săndulescu, O.; Popa, M.; Streinu-Cercel, A.; Alavidze, Z.; Berciu, I.; Bleotu, C.; Popa, M.I.; Chifiriuc, M.C.; Streinu-Cercel, A. Experimental Approach for Bacteriophage Susceptibility Testing of Planktonic and Sessile Bacterial Populations—Study Protocol. GERMS 2014, 4, 92-96. https://doi.org/10.11599/germs.2014.1062

AMA Style

Neguț AC, Săndulescu O, Popa M, Streinu-Cercel A, Alavidze Z, Berciu I, Bleotu C, Popa MI, Chifiriuc MC, Streinu-Cercel A. Experimental Approach for Bacteriophage Susceptibility Testing of Planktonic and Sessile Bacterial Populations—Study Protocol. GERMS. 2014; 4(4):92-96. https://doi.org/10.11599/germs.2014.1062

Chicago/Turabian Style

Neguț, Alina Cristina, Oana Săndulescu, Marcela Popa, Anca Streinu-Cercel, Zemphira Alavidze, Ioana Berciu, Coralia Bleotu, Mircea Ioan Popa, Mariana Carmen Chifiriuc, and Adrian Streinu-Cercel. 2014. "Experimental Approach for Bacteriophage Susceptibility Testing of Planktonic and Sessile Bacterial Populations—Study Protocol" GERMS 4, no. 4: 92-96. https://doi.org/10.11599/germs.2014.1062

APA Style

Neguț, A. C., Săndulescu, O., Popa, M., Streinu-Cercel, A., Alavidze, Z., Berciu, I., Bleotu, C., Popa, M. I., Chifiriuc, M. C., & Streinu-Cercel, A. (2014). Experimental Approach for Bacteriophage Susceptibility Testing of Planktonic and Sessile Bacterial Populations—Study Protocol. GERMS, 4(4), 92-96. https://doi.org/10.11599/germs.2014.1062

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