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GERMS
Volume 10
Issue 2
10.18683/germs.2020.1191
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Article

Molecular Surveillance of Methicillin-Susceptible Staphylococcus aureus (MSSA) Isolated over a One-Year Period from a Malaysian Teaching Hospital

by
Hassriana Fazilla Sapri
1,
Mohd Azrul Hisham Ismail
2,
Nurul Azirah Mohamad Sani
1,
Ainihayati Noordin
1,
Toh Leong Tan
3,
Salasawati Hussin
1,4 and
Hui-Min Neoh
2,*
1
Department of Medical Microbiology and Immunology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur 56000, Malaysia
2
UKM Medical Molecular Biology Institute (UMBI), Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur 56000, Malaysia
3
Department of Emergency Medicine, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur 56000, Malaysia
4
Medical Science Division, Faculty of Medicine, University of Cyberjaya, Cyberjaya 63000, Selangor, Malaysia
*
Author to whom correspondence should be addressed.
GERMS 2020, 10(2), 104-111; https://doi.org/10.18683/germs.2020.1191
Submission received: 14 February 2020 / Revised: 19 May 2020 / Accepted: 31 May 2020 / Published: 2 June 2020
Versions Notes

Abstract

Introduction: We report the results of a molecular surveillance study carried out on methicillin-susceptible Staphylococcus aureus (MSSA) isolated in a one-year duration from Hospital Canselor Tuanku Muhriz (HCTM), a tertiary hospital located in Kuala Lumpur, Malaysia. Methods: The first strain isolated from each MSSA infection in HCTM during the year 2009 was included into the study. Antimicrobial susceptibility testing and agr group typing were carried out for all strains; virulence gene (cna, seh, TSST-1 and PVL) typing results of the strains were obtained from a previous study. Pulsed-field gel electrophoresis (PFGE) was done on selected strains from the orthopedic ward. Relationship(s) between different typing methods used in the study was investigated, where a p value of <0.05 indicated significant association between typing methods. Results: A total of 880 MSSA strains were included into the study. The strains were generally susceptible to most antibiotics, with most of them carrying cna and agr-I (51.6%, n = 454; 39.8%, n = 350, respectively). A total of 17 PFGE pulsotypes were identified using an 80% similarity cut-off value, where the main pulsotype (pulsotype E) consisted of 24 isolates (23.5%). agr-III strains were found to be usually positive for both cna and seh (p < 0.05). In addition, some PFGE pulsotypes were also characteristic of certain virulence genes or agr groups. Conclusions: We did not identify a dominant MSSA clone circulating in HCTM in 2009. Nevertheless, results from this molecular surveillance will provide good baseline data for the hospital’s second S. aureus surveillance planned for the year 2020.
Keywords: molecular surveillance; methicillin-susceptible S. aureus (MSSA); agr typing; virulence gene typing; PFGE molecular surveillance; methicillin-susceptible S. aureus (MSSA); agr typing; virulence gene typing; PFGE

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MDPI and ACS Style

Sapri, H.F.; Ismail, M.A.H.; Sani, N.A.M.; Noordin, A.; Tan, T.L.; Hussin, S.; Neoh, H.-M. Molecular Surveillance of Methicillin-Susceptible Staphylococcus aureus (MSSA) Isolated over a One-Year Period from a Malaysian Teaching Hospital. GERMS 2020, 10, 104-111. https://doi.org/10.18683/germs.2020.1191

AMA Style

Sapri HF, Ismail MAH, Sani NAM, Noordin A, Tan TL, Hussin S, Neoh H-M. Molecular Surveillance of Methicillin-Susceptible Staphylococcus aureus (MSSA) Isolated over a One-Year Period from a Malaysian Teaching Hospital. GERMS. 2020; 10(2):104-111. https://doi.org/10.18683/germs.2020.1191

Chicago/Turabian Style

Sapri, Hassriana Fazilla, Mohd Azrul Hisham Ismail, Nurul Azirah Mohamad Sani, Ainihayati Noordin, Toh Leong Tan, Salasawati Hussin, and Hui-Min Neoh. 2020. "Molecular Surveillance of Methicillin-Susceptible Staphylococcus aureus (MSSA) Isolated over a One-Year Period from a Malaysian Teaching Hospital" GERMS 10, no. 2: 104-111. https://doi.org/10.18683/germs.2020.1191

APA Style

Sapri, H. F., Ismail, M. A. H., Sani, N. A. M., Noordin, A., Tan, T. L., Hussin, S., & Neoh, H.-M. (2020). Molecular Surveillance of Methicillin-Susceptible Staphylococcus aureus (MSSA) Isolated over a One-Year Period from a Malaysian Teaching Hospital. GERMS, 10(2), 104-111. https://doi.org/10.18683/germs.2020.1191

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