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Article

Biogenic Ferrihydrite Nanoparticles: Synthesis, Properties In Vitro and In Vivo Testing and the Concentration Effect

1
Federal Research Center KSC SB RAS, Kirensky Institute of Physics, 660036 Krasnoyarsk, Russia
2
Krasnoyarsk Scientific Center, Federal Research Center KSC SB RAS, 660036 Krasnoyarsk, Russia
3
Biophysics Department, Siberian Federal University, 660041 Krasnoyarsk, Russia
4
Federal Research Center KSC SB RAS, Scientific Research Institute of Medical Problems of the North, 660022 Krasnoyarsk, Russia
5
Federal Research Center KSC SB RAS, Institute of Biophysics, 660036 Krasnoyarsk, Russia
*
Author to whom correspondence should be addressed.
Academic Editor: Shaker A. Mousa
Biomedicines 2021, 9(3), 323; https://doi.org/10.3390/biomedicines9030323
Received: 4 February 2021 / Revised: 15 March 2021 / Accepted: 17 March 2021 / Published: 22 March 2021
(This article belongs to the Special Issue Molecular Mechanisms of Responses to Low-Intensity Exposures)
Biogenic ferrihydrite nanoparticles were synthesized as a result of the cultivation of Klebsiella oxytoca microorganisms. The distribution of nanoparticles in the body of laboratory animals and the physical properties of the nanoparticles were studied. The synthesized ferrihydrite nanoparticles are superparamagnetic at room temperature, and the characteristic blocking temperature is 23–25 K. The uncompensated moment of ferrihydrite particles was determined to be approximately 200 Bohr magnetons. In vitro testing of different concentrations of ferrihydrite nanoparticles for the functional activity of neutrophilic granulocytes by the chemiluminescence method showed an increase in the release of primary oxygen radicals by blood phagocytes when exposed to a minimum concentration and a decrease in secondary radicals when exposed to a maximum concentration. In vivo testing of ferrihydrite nanoparticles on Wister rats showed that a suspension of ferrihydrite nanoparticles has chronic toxicity, since it causes morphological changes in organs, mainly in the spleen, which are characterized by the accumulation of hemosiderin nanoparticles (stained blue according to Perls). Ferrihydrite can also directly or indirectly stimulate the proliferation and intracellular regeneration of hepatocytes. The partial detection of Perls-positive cells in the liver and kidneys can be explained by the rapid elimination from organs and the high dispersion of the nanomaterial. Thus, it is necessary to carry out studies of these processes at the systemic level, since the introduction of nanoparticles into the body is characterized by adaptive-proliferative processes, accompanied by the development of cell dystrophy and tension of the phagocytic system. View Full-Text
Keywords: ferrihydrite nanoparticles; concentration effect; microorganisms Klebsiella oxytoca; neutrophilic granulocytes; chemiluminescence; toxicology ferrihydrite nanoparticles; concentration effect; microorganisms Klebsiella oxytoca; neutrophilic granulocytes; chemiluminescence; toxicology
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MDPI and ACS Style

Stolyar, S.V.; Kolenchukova, O.A.; Boldyreva, A.V.; Kudryasheva, N.S.; Gerasimova, Y.V.; Krasikov, A.A.; Yaroslavtsev, R.N.; Bayukov, O.A.; Ladygina, V.P.; Birukova, E.A. Biogenic Ferrihydrite Nanoparticles: Synthesis, Properties In Vitro and In Vivo Testing and the Concentration Effect. Biomedicines 2021, 9, 323. https://doi.org/10.3390/biomedicines9030323

AMA Style

Stolyar SV, Kolenchukova OA, Boldyreva AV, Kudryasheva NS, Gerasimova YV, Krasikov AA, Yaroslavtsev RN, Bayukov OA, Ladygina VP, Birukova EA. Biogenic Ferrihydrite Nanoparticles: Synthesis, Properties In Vitro and In Vivo Testing and the Concentration Effect. Biomedicines. 2021; 9(3):323. https://doi.org/10.3390/biomedicines9030323

Chicago/Turabian Style

Stolyar, Sergey V., Oksana A. Kolenchukova, Anna V. Boldyreva, Nadezda S. Kudryasheva, Yulia V. Gerasimova, Alexandr A. Krasikov, Roman N. Yaroslavtsev, Oleg A. Bayukov, Valentina P. Ladygina, and Elena A. Birukova 2021. "Biogenic Ferrihydrite Nanoparticles: Synthesis, Properties In Vitro and In Vivo Testing and the Concentration Effect" Biomedicines 9, no. 3: 323. https://doi.org/10.3390/biomedicines9030323

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