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Article

Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template

1
Biomaterials for Regenerative Therapies Group, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, Spain
2
Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28040 Madrid, Spain
3
IMEM-BRT Group, Department of Material Science, Escola d’Enginyeria de Barcelona Est (EEBE), Technical University of Catalonia (UPC), 08019 Barcelona, Spain
4
Bioengineering Institute of Technology, Department of Basic Science, Universitat Internacional de Catalunya (UIC), 08195 Barcelona, Spain
*
Authors to whom correspondence should be addressed.
The two authors equally contributed to the manuscript.
Academic Editor: Anand Singh
Biomedicines 2021, 9(3), 232; https://doi.org/10.3390/biomedicines9030232
Received: 27 January 2021 / Revised: 15 February 2021 / Accepted: 22 February 2021 / Published: 25 February 2021
(This article belongs to the Special Issue Bioengineered In Vitro Models for Biomedical Applications)
Tissue engineering and regenerative medicine approaches use biomaterials in combination with cells to regenerate lost functions of tissues and organs to prevent organ transplantation. However, most of the current strategies fail in mimicking the tissue’s extracellular matrix properties. In order to mimic native tissue conditions, we developed cell-derived matrix (CDM) microtissues (MT). Our methodology uses poly-lactic acid (PLA) and Cultispher® S microcarriers’ (MCs’) as scaffold templates, which are seeded with rat bone marrow mesenchymal stem cells (rBM-MSCs). The scaffold template allows cells to generate an extracellular matrix, which is then extracted for downstream use. The newly formed CDM provides cells with a complex physical (MT architecture) and biochemical (deposited ECM proteins) environment, also showing spontaneous angiogenic potential. Our results suggest that MTs generated from the combination of these two MCs (mixed MTs) are excellent candidates for tissue vascularization. Overall, this study provides a methodology for in-house fabrication of microtissues with angiogenic potential for downstream use in various tissue regenerative strategies. View Full-Text
Keywords: poly-lactic acid microcarriers; Cultispher® S; rat bone marrow mesenchymal stem cells; microtissue; cell-derived matrix; angiogenesis poly-lactic acid microcarriers; Cultispher® S; rat bone marrow mesenchymal stem cells; microtissue; cell-derived matrix; angiogenesis
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MDPI and ACS Style

Rubí-Sans, G.; Cano-Torres, I.; Pérez-Amodio, S.; Blanco-Fernandez, B.; Mateos-Timoneda, M.A.; Engel, E. Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template. Biomedicines 2021, 9, 232. https://doi.org/10.3390/biomedicines9030232

AMA Style

Rubí-Sans G, Cano-Torres I, Pérez-Amodio S, Blanco-Fernandez B, Mateos-Timoneda MA, Engel E. Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template. Biomedicines. 2021; 9(3):232. https://doi.org/10.3390/biomedicines9030232

Chicago/Turabian Style

Rubí-Sans, Gerard, Irene Cano-Torres, Soledad Pérez-Amodio, Barbara Blanco-Fernandez, Miguel A. Mateos-Timoneda, and Elisabeth Engel. 2021. "Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template" Biomedicines 9, no. 3: 232. https://doi.org/10.3390/biomedicines9030232

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