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Article

Lobeglitazone Exerts Anti-Inflammatory Effect in Lipopolysaccharide-Induced Bone-Marrow Derived Macrophages

1
Department of Neurosurgery, CHA Bundang Medical Center, Seongnam-si 13496, Korea
2
Department of Biology, Lawrence University, Appleton, WI 54911, USA
3
Department of Biomedical Science, CHA University, Seongnam-si 13493, Korea
*
Authors to whom correspondence should be addressed.
Academic Editor: Alexander N. Orekhov
Biomedicines 2021, 9(10), 1432; https://doi.org/10.3390/biomedicines9101432
Received: 30 August 2021 / Revised: 5 October 2021 / Accepted: 7 October 2021 / Published: 10 October 2021
(This article belongs to the Special Issue Macrophages in Health and Non-infectious Disease 2.0)
The purpose of this study is to elucidate the anti-inflammatory effect of lobeglitazone (LOBE) in lipopolysaccharide (LPS)-induced bone-marrow derived macrophages (BMDMs). We induced nitric oxide (NO) production and pro-inflammatory gene expression through LPS treatment in BMDMs. The changes of NO release and expression of pro-inflammatory mediators by LOBE were assessed via NO quantification assay and a real-time quantitative polymerase chain reaction (RT-qPCR), respectively. In addition, the regulatory effect of LOBE on activation of mitogen-activated protein kinase (MAPK) signaling pathway was investigated by measuring the phosphorylation state of extracellular regulatory protein (ERK) and c-Jun N-terminal kinase (JNK) proteins by Western blot. Our results show that LOBE significantly reduced LPS-induced NO production and pro-inflammatory gene expression of interleukin-1β (IL-1β), IL-6, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and monocyte chemoattractant protein-1 (MCP-1). Moreover, LOBE reduced phosphorylation levels of ERK and JNK of MAPK signaling pathway. In conclusion, LOBE exerts an anti-inflammatory effect in LPS-induced BMDMs by suppression of NO production and pro-inflammatory gene expression, and this effect is potentially through inhibition of the MARK signaling pathway. View Full-Text
Keywords: bone-marrow derived macrophages; lipopolysaccharide; lobeglitazone; anti-inflammation; mitogen-activated protein kinase bone-marrow derived macrophages; lipopolysaccharide; lobeglitazone; anti-inflammation; mitogen-activated protein kinase
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MDPI and ACS Style

Jeong, D.; Ko, W.-K.; Kim, S.-J.; Han, G.-H.; Lee, D.; Sheen, S.-H.; Sohn, S. Lobeglitazone Exerts Anti-Inflammatory Effect in Lipopolysaccharide-Induced Bone-Marrow Derived Macrophages. Biomedicines 2021, 9, 1432. https://doi.org/10.3390/biomedicines9101432

AMA Style

Jeong D, Ko W-K, Kim S-J, Han G-H, Lee D, Sheen S-H, Sohn S. Lobeglitazone Exerts Anti-Inflammatory Effect in Lipopolysaccharide-Induced Bone-Marrow Derived Macrophages. Biomedicines. 2021; 9(10):1432. https://doi.org/10.3390/biomedicines9101432

Chicago/Turabian Style

Jeong, Dabin, Wan-Kyu Ko, Seong-Jun Kim, Gong-Ho Han, Daye Lee, Seung-Hun Sheen, and Seil Sohn. 2021. "Lobeglitazone Exerts Anti-Inflammatory Effect in Lipopolysaccharide-Induced Bone-Marrow Derived Macrophages" Biomedicines 9, no. 10: 1432. https://doi.org/10.3390/biomedicines9101432

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