Anti-Cancer Activity of the Combinational Treatment of Noozone Cold Plasma with p-FAK Antibody-Conjugated Gold Nanoparticles in OSCC Xenograft Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Immunofluorescence
2.3. Western Blot Analysis
2.4. Formation of p-FAK/GNP Conjugates and Its Treatment
2.5. NCP Device
2.6. Live/Dead Cell Staining
2.7. Animal Experiments
2.8. Anti-OSCC Activity Evaluation in a Mice Xenograft Model
2.9. Histological Assays
2.10. Statistical Analysis
3. Results
3.1. Phosphorylated FAK Protein Was Highly Expressed in OSCC Cells
3.2. The Combinational Treatment of NCP with p-FAK/GNP Selectively Kills SCC25 Cells Immediately after Treatment
3.3. The Effects of Plasma-Activated Media Treatment on p-FAK/GNP-Treated SCC25 Cells Was Weaker Than the Direct NCP Treatment
3.4. The Placement of Electronic Grounded Mesh during NCP Treatment Hindersp-FAK/GNP+NCP-Mediated SCC25 Cell Death
3.5. The Synergistic Anti-Tumor Effect of the Combinational Treatment of NCP and p-FAK/GNP in Mice Xenograft Models
3.6. The Tissue Isolated from the Mice Treated with pFAK/GNP in Combination with NCP Was Not a Tumor
3.7. Apoptotic Cell Death Was Not Monitored in the Tumors Treated with NCP and p-FAK/GNP in Combination
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
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Choi, J.-H.; Gu, H.-J.; Park, K.-H.; Hwang, D.-S.; Kim, G.-C. Anti-Cancer Activity of the Combinational Treatment of Noozone Cold Plasma with p-FAK Antibody-Conjugated Gold Nanoparticles in OSCC Xenograft Mice. Biomedicines 2022, 10, 2259. https://doi.org/10.3390/biomedicines10092259
Choi J-H, Gu H-J, Park K-H, Hwang D-S, Kim G-C. Anti-Cancer Activity of the Combinational Treatment of Noozone Cold Plasma with p-FAK Antibody-Conjugated Gold Nanoparticles in OSCC Xenograft Mice. Biomedicines. 2022; 10(9):2259. https://doi.org/10.3390/biomedicines10092259
Chicago/Turabian StyleChoi, Jeong-Hae, Hee-Jin Gu, Kwang-Ha Park, Dae-Seok Hwang, and Gyoo-Cheon Kim. 2022. "Anti-Cancer Activity of the Combinational Treatment of Noozone Cold Plasma with p-FAK Antibody-Conjugated Gold Nanoparticles in OSCC Xenograft Mice" Biomedicines 10, no. 9: 2259. https://doi.org/10.3390/biomedicines10092259