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Open AccessArticle

Microarray Selection of Cooperative Peptides for Modulating Enzyme Activities

by Jinglin Fu 1,2
1
Department of Chemistry, Rutgers University-Camden, Camden, NJ 08102, USA
2
Center for Computational and Integrative Biology, Rutgers University-Camden, Camden, NJ 08102, USA
Academic Editor: Vehary Sakanyan
Microarrays 2017, 6(2), 8; https://doi.org/10.3390/microarrays6020008
Received: 17 March 2017 / Revised: 21 April 2017 / Accepted: 24 April 2017 / Published: 26 April 2017
(This article belongs to the Special Issue Microarrays for Drug Discovery and Development)
Recently, peptide microarrays have been used to distinguish proteins, antibodies, viruses, and bacteria based on their binding to random sequence peptides. We reported on the use of peptide arrays to identify enzyme modulators that involve screening an array of 10,000 defined and addressable peptides on a microarray. Primary peptides were first selected to inhibit the enzyme at low μM concentrations. Then, new peptides were found to only bind strongly with the enzyme–inhibitor complex, but not the native enzyme. These new peptides served as secondary inhibitors that enhanced the inhibition of the enzyme together with the primary peptides. Without the primary peptides, the secondary effect peptides had little effect on the enzyme activity. Conversely, we also selected peptides that recovered the activities of inhibited enzyme–peptide complex. The selection of cooperative peptide pairs will provide a versatile toolkit for modulating enzyme functions, which may potentially be applied to drug discovery and biocatalysis. View Full-Text
Keywords: microarray; peptides; enzyme inhibition; β-galactosidase microarray; peptides; enzyme inhibition; β-galactosidase
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Fu, J. Microarray Selection of Cooperative Peptides for Modulating Enzyme Activities. Microarrays 2017, 6, 8.

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