Next Article in Journal / Special Issue
Qualitative and Quantitative Requirements for Assessing Prognostic Markers in Prostate Cancer
Previous Article in Journal / Special Issue
Identification of New Players in Hepatocarcinogenesis: Limits and Opportunities of Using Tissue Microarray (TMA)
Article Menu

Export Article

From the third issue of 2017, Microarrays has changed its name to High-Throughput.

Open AccessReview

Overview on Techniques to Construct Tissue Arrays with Special Emphasis on Tissue Microarrays

Institute of Pathology, University Hospital, Eberhard-Karls-University, Liebermeisterstrasse 8, 72076 Tuebingen, Germany
Microarrays 2014, 3(2), 103-136;
Received: 9 January 2014 / Revised: 28 March 2014 / Accepted: 9 April 2014 / Published: 17 April 2014
PDF [2289 KB, uploaded 17 April 2014]


With the advent of new histopathological staining techniques (histochemistry, immunohistochemistry, in situ hybridization) and the discovery of thousands of new genes, mRNA, and proteins by molecular biology, the need grew for a technique to compare many different cells or tissues on one slide in a cost effective manner and with the possibility to easily track the identity of each specimen: the tissue array (TA). Basically, a TA consists of at least two different specimens per slide. TAs differ in the kind of specimens, the number of specimens installed, the dimension of the specimens, the arrangement of the specimens, the embedding medium, the technique to prepare the specimens to be installed, and the technique to construct the TA itself. A TA can be constructed by arranging the tissue specimens in a mold and subsequently pouring the mold with the embedding medium of choice. In contrast, preformed so-called recipient blocks consisting of the embedding medium of choice have punched, drilled, or poured holes of different diameters and distances in which the cells or tissue biopsies will be deployed manually, semi-automatically, or automatically. The costs of constructing a TA differ from a few to thousands of Euros depending on the technique/equipment used. Remarkably high quality TAs can be also achieved by low cost techniques. View Full-Text
Keywords: tissue microarrays; techniques; paraffin; OCT tissue microarrays; techniques; paraffin; OCT
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Vogel, U. Overview on Techniques to Construct Tissue Arrays with Special Emphasis on Tissue Microarrays. Microarrays 2014, 3, 103-136.

Show more citation formats Show less citations formats

Article Metrics

Article Access Statistics



[Return to top]
Microarrays EISSN 2076-3905 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top