Special Issue "Recent CMV Research"
Deadline for manuscript submissions: 31 December 2013
Prof. Dr. Anamaris M. Colberg-Poley
Professor of Pediatrics, Integrative Systems Biology and of Biochemistry/Molecular Biology George Washington University School of Medicine, Senior Investigator, Research Center for Genetic Medicine, Room M5110 Children's National Medical Center, 111 Michigan Ave., NW Washington, DC 20010, USA
Phone: +1 202 476 3984
Fax: +1 202 476 6014
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. Papers will be published continuously (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are refereed through a peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Viruses is an international peer-reviewed Open Access monthly journal published by MDPI.
Viruses 2013, 5(11), 2803-2824; doi:10.3390/v5112803
Received: 17 October 2013; in revised form: 11 November 2013 / Accepted: 13 November 2013 / Published: 21 November 2013| Download PDF Full-text (595 KB) | Download XML Full-text
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Title: Analysis of essential viral gene functions after highly efficient adenofection of cells with cloned human cytomegalovirus genomes
Authors: Endrit Elbasani, Ildar Gabaev, Lars Steinbrück, Martin Messerle and Eva Maria Borst*
Affiliation: Department of Virology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany; E-Mails: firstname.lastname@example.org, email@example.com, firstname.lastname@example.org, email@example.com and firstname.lastname@example.org
Abstract: Human cytomegalovirus (HCMV) has a large 240 kb genome that may encode more than 700 gene products with many of them remaining uncharacterized. Mutagenesis of bacterial artificial chromosome (BAC)-cloned CMV genomes has greatly facilitated the analysis of viral gene functions, yet especially the roles of essential proteins often remain elusive, because their investigation required the cumbersome establishment of suitable complementation systems. Here, we show that HCMV genomes can be introduced into cells with unprecedented efficiency by applying a transfection protocol based on replication-defective, inactivated adenovirus particles (adenofection). Upon adenofection of several permissive cell types with HCMV genomes carrying mutations in essential genes transfection rates of up to 60% were observed and viral proteins of all kinetic classes were found to be expressed. This enabled further analyses of the transfected cells by well-established biochemical techniques. Remarkably, HCMV genomes lacking elements essential for viral DNA replication such as the lytic origin of replication still expressed several late proteins. In conclusion, adenofection allows to study essential HCMV genes directly in BAC-transfected cells without the need for sophisticated complementation strategies.
Last update: 18 November 2013