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Viruses 2014, 6(1), 172-188; doi:10.3390/v6010172

Proteomic Analyses of Human Cytomegalovirus Strain AD169 Derivatives Reveal Highly Conserved Patterns of Viral and Cellular Proteins in Infected Fibroblasts

1
Institute for Virology, University Medical Center of the Johannes Gutenberg-University Mainz, Obere Zahlbacher Str. 67, D-55131 Mainz, Germany
2
Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Langenbeckstr. 1, D-55131 Mainz, Germany
3
Research Center Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Langenbeckstr. 1, D-55131 Mainz, Germany
*
Author to whom correspondence should be addressed.
Received: 15 November 2013 / Revised: 29 December 2013 / Accepted: 30 December 2013 / Published: 7 January 2014
(This article belongs to the Special Issue Recent CMV Research)
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Abstract

Human cytomegalovirus (HCMV) particle morphogenesis in infected cells is an orchestrated process that eventually results in the release of enveloped virions. Proteomic analysis has been employed to reveal the complexity in the protein composition of these extracellular particles. Only limited information is however available regarding the proteome of infected cells preceding the release of HCMV virions. We used quantitative mass spectrometry to address the pattern of viral and cellular proteins in cells, infected with derivatives of the AD169 laboratory strain. Our analyses revealed a remarkable conservation in the patterns of viral and of abundant cellular proteins in cells, infected for 2 hours, 2 days, or 4 days. Most viral proteins increased in abundance as the infection progressed over time. Of the proteins that were reliably detectable by mass spectrometry, only IE1 (pUL123), pTRS1, and pIRS1 were downregulated at 4 days after infection. In addition, little variation of viral proteins in the virions of the different viruses was detectable, independent of the expression of the major tegument protein pp65. Taken together these data suggest that there is little variation in the expression program of viral and cellular proteins in cells infected with related HCMVs, resulting in a conserved pattern of viral proteins ultimately associated with extracellular virions. View Full-Text
Keywords: human cytomegalovirus; proteomics; mass spectrometry; virions; expression pattern human cytomegalovirus; proteomics; mass spectrometry; virions; expression pattern
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Reyda, S.; Büscher, N.; Tenzer, S.; Plachter, B. Proteomic Analyses of Human Cytomegalovirus Strain AD169 Derivatives Reveal Highly Conserved Patterns of Viral and Cellular Proteins in Infected Fibroblasts. Viruses 2014, 6, 172-188.

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