Viruses 2014, 6(3), 1049-1072; doi:10.3390/v6031049
Review

Genomic and Functional Characteristics of Human Cytomegalovirus Revealed by Next-Generation Sequencing

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Received: 20 January 2014; in revised form: 11 February 2014 / Accepted: 11 February 2014 / Published: 5 March 2014
(This article belongs to the Special Issue Recent CMV Research)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The complete genome of human cytomegalovirus (HCMV) was elucidated almost 25 years ago using a traditional cloning and Sanger sequencing approach. Analysis of the genetic content of additional laboratory and clinical isolates has lead to a better, albeit still incomplete, definition of the coding potential and diversity of wild-type HCMV strains. The introduction of a new generation of massively parallel sequencing technologies, collectively called next-generation sequencing, has profoundly increased the throughput and resolution of the genomics field. These increased possibilities are already leading to a better understanding of the circulating diversity of HCMV clinical isolates. The higher resolution of next-generation sequencing provides new opportunities in the study of intrahost viral population structures. Furthermore, deep sequencing enables novel diagnostic applications for sensitive drug resistance mutation detection. RNA-seq applications have changed the picture of the HCMV transcriptome, which resulted in proof of a vast amount of splicing events and alternative transcripts. This review discusses the application of next-generation sequencing technologies, which has provided a clearer picture of the intricate nature of the HCMV genome. The continuing development and application of novel sequencing technologies will further augment our understanding of this ubiquitous, but elusive, herpesvirus.
Keywords: cytomegalovirus; genomics; transcriptomics; next-generation sequencing; drug resistance; deep sequencing
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MDPI and ACS Style

Sijmons, S.; Van Ranst, M.; Maes, P. Genomic and Functional Characteristics of Human Cytomegalovirus Revealed by Next-Generation Sequencing. Viruses 2014, 6, 1049-1072.

AMA Style

Sijmons S, Van Ranst M, Maes P. Genomic and Functional Characteristics of Human Cytomegalovirus Revealed by Next-Generation Sequencing. Viruses. 2014; 6(3):1049-1072.

Chicago/Turabian Style

Sijmons, Steven; Van Ranst, Marc; Maes, Piet. 2014. "Genomic and Functional Characteristics of Human Cytomegalovirus Revealed by Next-Generation Sequencing." Viruses 6, no. 3: 1049-1072.

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