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Viruses 2014, 6(2), 727-751; doi:10.3390/v6020727
Article

Identification by Mass Spectrometry and Immune Response Analysis of Guinea Pig Cytomegalovirus (GPCMV) Pentameric Complex Proteins GP129, 131 and 133

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Received: 18 November 2013 / Revised: 3 January 2014 / Accepted: 14 January 2014 / Published: 13 February 2014
(This article belongs to the Special Issue Recent CMV Research)
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Abstract

Development of a vaccine against congenital infection with human cytomegalovirus (HCMV) is a major public health priority. A potential vaccine target receiving considerable recent attention is the pentameric complex (PC) of HCMV proteins consisting of gL, gH, UL128, UL130, and UL131, since some antibodies against these target proteins are capable of potently neutralizing virus at epithelial and endothelial cell surfaces. Recently, homologous proteins have been described for guinea pig cytomegalovirus (GPCMV), consisting of gH, gL, and the GPCMV proteins GP129, GP131, and GP133. To investigate these proteins as potential vaccine targets, expression of GP129-GP133 transcripts was confirmed by reverse-transcriptase PCR. Mass spectrometry combined with western blot assays demonstrated the presence of GP129, GP131, and GP133 proteins in virus particles. Recombinant proteins corresponding to these PC proteins were generated in baculovirus, and as GST fusion proteins. Recombinant proteins were noted to be immunoreactive with convalescent sera from infected animals, suggesting that these proteins are recognized in the humoral immune response to GPCMV infection. These analyses support the study of PC-based recombinant vaccines in the GPCMV congenital infection model.
Keywords: guinea pig cytomegalovirus; cytomegalovirus vaccine; pentameric complex; congenital cytomegalovirus infection; baculovirus guinea pig cytomegalovirus; cytomegalovirus vaccine; pentameric complex; congenital cytomegalovirus infection; baculovirus
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Gnanandarajah, J.S.; Gillis, P.A.; Hernandez-Alvarado, N.; Higgins, L.; Markowski, T.W.; Sung, H.; Lumley, S.; Schleiss, M.R. Identification by Mass Spectrometry and Immune Response Analysis of Guinea Pig Cytomegalovirus (GPCMV) Pentameric Complex Proteins GP129, 131 and 133. Viruses 2014, 6, 727-751.

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