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Viruses 2014, 6(2), 448-475; doi:10.3390/v6020448
Article

Molecular and Biological Characterization of a New Isolate of Guinea Pig Cytomegalovirus

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Received: 18 November 2013; in revised form: 9 January 2014 / Accepted: 9 January 2014 / Published: 27 January 2014
(This article belongs to the Special Issue Recent CMV Research)
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Abstract: Development of a vaccine against congenital infection with human cytomegalovirus is complicated by the issue of re-infection, with subsequent vertical transmission, in women with pre-conception immunity to the virus. The study of experimental therapeutic prevention of re-infection would ideally be undertaken in a small animal model, such as the guinea pig cytomegalovirus (GPCMV) model, prior to human clinical trials. However, the ability to model re-infection in the GPCMV model has been limited by availability of only one strain of virus, the 22122 strain, isolated in 1957. In this report, we describe the isolation of a new GPCMV strain, the CIDMTR strain. This strain demonstrated morphological characteristics of a typical Herpesvirinae by electron microscopy. Illumina and PacBio sequencing demonstrated a genome of 232,778 nt. Novel open reading frames ORFs not found in reference strain 22122 included an additional MHC Class I homolog near the right genome terminus. The CIDMTR strain was capable of dissemination in immune compromised guinea pigs, and was found to be capable of congenital transmission in GPCMV-immune dams previously infected with salivary gland‑adapted strain 22122 virus. The availability of a new GPCMV strain should facilitate study of re-infection in this small animal model.
Keywords: guinea pig cytomegalovirus; cytomegalovirus strain variation; CMV immune evasion; congenital cytomegalovirus infection; congenital CMV vaccines guinea pig cytomegalovirus; cytomegalovirus strain variation; CMV immune evasion; congenital cytomegalovirus infection; congenital CMV vaccines
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Schleiss, M.R.; McAllister, S.; Armién, A.G.; Hernandez-Alvarado, N.; Fernández-Alarcón, C.; Zabeli, J.C.; Ramaraj, T.; Crow, J.A.; McVoy, M.A. Molecular and Biological Characterization of a New Isolate of Guinea Pig Cytomegalovirus. Viruses 2014, 6, 448-475.

AMA Style

Schleiss MR, McAllister S, Armién AG, Hernandez-Alvarado N, Fernández-Alarcón C, Zabeli JC, Ramaraj T, Crow JA, McVoy MA. Molecular and Biological Characterization of a New Isolate of Guinea Pig Cytomegalovirus. Viruses. 2014; 6(2):448-475.

Chicago/Turabian Style

Schleiss, Mark R.; McAllister, Shane; Armién, Anibal G.; Hernandez-Alvarado, Nelmary; Fernández-Alarcón, Claudia; Zabeli, Jason C.; Ramaraj, Thiruvarangan; Crow, John A.; McVoy, Michael A. 2014. "Molecular and Biological Characterization of a New Isolate of Guinea Pig Cytomegalovirus." Viruses 6, no. 2: 448-475.


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