Special Issue "Single Molecule Techniques"
Deadline for manuscript submissions: closed (15 June 2014)
Dr. Hans-Heiner Gorris
Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitätsstrasse 31, 93040 Regensburg, Germany
Website | E-Mail
Interests: single molecule analysis in femtoliter arrays; enzyme kinetics; ultrasensitive and background-free detection of analytes; photon-upconverting nanoparticles
Single molecule experiments have unraveled processes that were previously concealed in bulk experiments because they were not synchronized or even heterogeneously distributed among individual molecules in a population. New insights into the function, structure and interactions of individual molecules have been driven by technological advances in single molecule manipulation and detection. Fluorescence spectroscopy is one of the most powerful single molecule techniques because it has a high spatial and temporal resolution and is very sensitive, as long as the background can be efficiently suppressed.
In biochemistry/biophysics, transitions or differences in the conformation of proteins have been observed as dynamic or static heterogeneity. Labeling of proteins and DNA with donor-quencher pairs has enabled the detection of binding interactions by fluorescence resonance energy transfer (FRET). Site-specific labeling of protein subunits has enabled the use of FRET as a nanoscale ruler for measuring conformational transitions. On the other hand, fluorogenic substrates can be employed for investigating the catalytic activity of single enzyme molecules. The analysis of single molecule binding and transition events has entailed a shift from a kinetic to a stochastic perspective on biochemical processes.
This Special Issue of Molecules covers all aspects related to the development and the application of "Single Molecule Techniques". Not only are techniques based on fluorescence spectroscopy welcome but also those based on atomic force spectroscopy, scanning-tunneling electron microscopy or others. It is a pleasure to invite original research as well as review articles that describe and discuss technical developments in the detection and manipulation of single molecules as well as their applications.
Dr. Hans-H. Gorris
Manuscript Submission Information
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- conformational dynamics
- dynamic/static heterogeneity
- fluorescence resonance energy transfer (FRET)
- fluorescence spectroscopy
- single molecules
- super-resolution microscopy