Special Issue "Single Molecule Techniques"


A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Molecular Diversity".

Deadline for manuscript submissions: closed (15 June 2014)

Special Issue Editor

Guest Editor
Dr. Hans-Heiner Gorris
Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitätsstrasse 31, 93040 Regensburg, Germany
Website: http://www-analytik.chemie.uni-regensburg.de/wolfbeis/gorris.htm
E-Mail: hans-heiner.gorris@ur.de
Interests: single molecule analysis in femtoliter arrays; enzyme kinetics; ultrasensitive and background-free detection of analytes; photon-upconverting nanoparticles

Special Issue Information

Dear Colleagues,

Single molecule experiments have unraveled processes that were previously concealed in bulk experiments because they were not synchronized or even heterogeneously distributed among individual molecules in a population. New insights into the function, structure and interactions of individual molecules have been driven by technological advances in single molecule manipulation and detection. Fluorescence spectroscopy is one of the most powerful single molecule techniques because it has a high spatial and temporal resolution and is very sensitive, as long as the background can be efficiently suppressed.

In biochemistry/biophysics, transitions or differences in the conformation of proteins have been observed as dynamic or static heterogeneity. Labeling of proteins and DNA with donor-quencher pairs has enabled the detection of binding interactions by fluorescence resonance energy transfer (FRET). Site-specific labeling of protein subunits has enabled the use of FRET as a nanoscale ruler for measuring conformational transitions. On the other hand, fluorogenic substrates can be employed for investigating the catalytic activity of single enzyme molecules. The analysis of single molecule binding and transition events has entailed a shift from a kinetic to a stochastic perspective on biochemical processes.

This Special Issue of Molecules covers all aspects related to the development and the application of "Single Molecule Techniques". Not only are techniques based on fluorescence spectroscopy welcome but also those based on atomic force spectroscopy, scanning-tunneling electron microscopy or others. It is a pleasure to invite original research as well as review articles that describe and discuss technical developments in the detection and manipulation of single molecules as well as their applications.

Dr. Hans-H. Gorris
Guest Editor


Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. Papers will be published continuously (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are refereed through a peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed Open Access monthly journal published by MDPI.

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  • biochemistry/biophysics
  • conformational dynamics
  • DNA
  • dynamic/static heterogeneity
  • fluorescence resonance energy transfer (FRET)
  • fluorescence spectroscopy
  • proteins
  • single molecules
  • super-resolution microscopy

Published Papers (3 papers)

by , , , , ,  and
Molecules 2014, 19(8), 12531-12546; doi:10.3390/molecules190812531
Received: 16 June 2014; in revised form: 31 July 2014 / Accepted: 7 August 2014 / Published: 18 August 2014
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by , ,  and
Molecules 2014, 19(8), 12116-12149; doi:10.3390/molecules190812116
Received: 24 June 2014; in revised form: 1 August 2014 / Accepted: 1 August 2014 / Published: 13 August 2014
Show/Hide Abstract | PDF Full-text (6136 KB) | XML Full-text
abstract graphic

by , ,  and
Molecules 2014, 19(8), 11660-11678; doi:10.3390/molecules190811660
Received: 4 July 2014; in revised form: 23 July 2014 / Accepted: 25 July 2014 / Published: 6 August 2014
Show/Hide Abstract | PDF Full-text (7038 KB) | XML Full-text
abstract graphic

Last update: 18 July 2014

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