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Molecules 2014, 19(8), 11660-11678; https://doi.org/10.3390/molecules190811660

Tracking Electrons in Biological Macromolecules: From Ensemble to Single Molecule

1
Commissariat à l'Energie Atomique, Institut de Biologie et de Technologies de Saclay, Service de Bioénergétique, Biologie Structurale et Mécanismes (CNRS UMR-8221), Gif-sur-Yvette Cedex 91191, France
2
Leiden Institute of Physics, Huygens-Kamerlingh Onnes Laboratory, Leiden University, Niels Bohrweg 2, PO Box 9504, RA Leiden 2300, The Netherlands
*
Author to whom correspondence should be addressed.
Received: 4 July 2014 / Revised: 23 July 2014 / Accepted: 25 July 2014 / Published: 6 August 2014
(This article belongs to the Special Issue Single Molecule Techniques)
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Abstract

Nature utilizes oxido-reductases to cater to the energy demands of most biochemical processes in respiratory species. Oxido-reductases are capable of meeting this challenge by utilizing redox active sites, often containing transition metal ions, which facilitate movement and relocation of electrons/protons to create a potential gradient that is used to energize redox reactions. There has been a consistent struggle by researchers to estimate the electron transfer rate constants in physiologically relevant processes. This review provides a brief background on the measurements of electron transfer rates in biological molecules, in particular Cu-containing enzymes, and highlights the recent advances in monitoring these electron transfer events at the single molecule level or better to say, at the individual event level. View Full-Text
Keywords: electron transfer; random sequential; single molecule; enzyme; FRET; redox; nitrite reductase; small laccase; copper protein; ABEL trap electron transfer; random sequential; single molecule; enzyme; FRET; redox; nitrite reductase; small laccase; copper protein; ABEL trap
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Tabares, L.C.; Gupta, A.; Aartsma, T.J.; Canters, G.W. Tracking Electrons in Biological Macromolecules: From Ensemble to Single Molecule. Molecules 2014, 19, 11660-11678.

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