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Molecules 2014, 19(9), 13803-13823; doi:10.3390/molecules190913803

Molecular Processes Studied at a Single-Molecule Level Using DNA Origami Nanostructures and Atomic Force Microscopy

1,2,* and 3,*
1
Institute of Chemistry—Physical Chemistry, Universität Potsdam, Karl-Liebknecht-Straße 24-25, D-14476 Potsdam, Germany
2
BAM Federal Institute of Materials Research and Testing, Richard-Willstätter Str. 11, D-12489 Berlin, Germany
3
Technical and Macromolecular Chemistry, University of Paderborn, Warburger Str. 100, D-33098 Paderborn, Germany
*
Authors to whom correspondence should be addressed.
Received: 23 July 2014 / Revised: 21 August 2014 / Accepted: 29 August 2014 / Published: 3 September 2014
(This article belongs to the Special Issue Single Molecule Techniques)
View Full-Text   |   Download PDF [14040 KB, uploaded 10 September 2014]   |  

Abstract

DNA origami nanostructures allow for the arrangement of different functionalities such as proteins, specific DNA structures, nanoparticles, and various chemical modifications with unprecedented precision. The arranged functional entities can be visualized by atomic force microscopy (AFM) which enables the study of molecular processes at a single-molecular level. Examples comprise the investigation of chemical reactions, electron-induced bond breaking, enzymatic binding and cleavage events, and conformational transitions in DNA. In this paper, we provide an overview of the advances achieved in the field of single-molecule investigations by applying atomic force microscopy to functionalized DNA origami substrates. View Full-Text
Keywords: DNA origami; atomic force microscopy; single-molecule analysis; DNA radiation damage; protein binding; enzyme reactions; G quadruplexes DNA origami; atomic force microscopy; single-molecule analysis; DNA radiation damage; protein binding; enzyme reactions; G quadruplexes
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Bald, I.; Keller, A. Molecular Processes Studied at a Single-Molecule Level Using DNA Origami Nanostructures and Atomic Force Microscopy. Molecules 2014, 19, 13803-13823.

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