Special Issue "FRET Biosensors"
Deadline for manuscript submissions: closed (30 June 2016)
Prof. Dr. Niko Hildebrandt (Website)
NanoBioPhotonics, Institut d’Electronique Fondamentale, Université Paris-Sud 91405 Orsay Cedex, France
Interests: FRET; spectroscopy; imaging; diagnostics; immunoassays; multiplexing; biosensing; lanthanides; quantum dots; time-gating
Dr. Igor Medintz (Website)
Laboratory for Biosensors and Biomaterials, Center for Biomolecular Science & Engineering, United States Naval Research Laboratory, Washington, D.C. USA
Fax: +1 202 7679594
Interests: nanoparticle-biological interface, energy transfer, FRET, biosensing, enzymatic catalysis at a nanoparticle interface, nanoparticle-based cellular imaging
Prof. Dr. Russ Algar (Website)
Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, BC Canada V6T 1Z1, Canada
Interests: fluorescence; resonance energy transfer; assays, imaging; biosensing; point-of-care diagnostics; nanoparticles; enzymes; nucleic acids; surface chemistry
FRET or Förster resonance energy transfer is a versatile and sensitive tool for qualitative and quantitative analysis of biological interactions and processes. The access to a wide range of fluorescent materials, in conjunction with improved, easy-to-use, and yet very sophisticated microscopes and spectrometers, have made FRET a very prominent technique for biosensing. Fluorophores that are utilized in FRET now encompass organic dyes, fluorescent proteins, semiconductor quantum dots, metal chelates, various noble metal and other nanoparticles, intrinsically fluorescent amino acids, biological cofactors, and polymers, to name but a few members of this growing library. Hand-in-hand with materials development is the growing availability of numerous reactive and bioorthogonal chemistries to specifically attach such fluorophores to all types of biological molecules, ranging from proteins to DNA. The unique ability of FRET to probe nanoscale inter- and intramolecular separation distances, has also led to a rapidly growing field of structural FRET studies of biomolecules and biological complexes.
We invite manuscripts for this forthcoming Special Issue that describe all aspects pertinent to FRET-based biosensing and bioimaging. Both reviews and original research articles will be published. Reviews should provide an up-to-date and critical overview of the current state of the art in a particular application, such as diagnostics and protein–protein interactions, or a particular technique such as single-molecule FRET or FRET spectroscopic rulers. Original research papers that describe the utilization of FRET in biosensing, or new concepts and fundamental studies with potential relevance to biosensing, are also of interest. If you have a preliminary idea or suggestion you would like to discuss beforehand, please feel free to contact us. We look forward to and welcome your participation in this Special Issue.
Prof. Dr. Niko Hildebrandt
Dr. Igor Medintz
Prof. Dr. Russ Algar
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. Papers will be published continuously (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are refereed through a peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Sensors is an international peer-reviewed Open Access monthly journal published by MDPI.
- fluorescent probes
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Title: Towards cellular imaging of Ca2+ and H+ microdomains
Author: Alsu I. Zamaleeva, 1,2,3,*, Despras Guillaume, 4,5,6, Camilla Luccardini, 1,2,3, Mayeul Collot, 4,5,6, Martin Oheim 7,8, Michel De Waard 9,10, Jean-Maurice Mallet 4,5,6, Anne Feltz 1,2,3,*
1Ecole Normale Supérieure, Institut de Biologie de l'ENS (IBENS), Paris F-75005, France
2 INSERM U1024, Paris F-75005, France
3 CNRS UMR 8197, Paris F-75005, France
4 UPMC Université́ Paris 06, Ecole Normale Supérieure (ENS), Paris, F-75005 France
5 CNRS UMR 7203, Paris F-75005, France
6 Laboratory of Biomolecules (LBM), Paris F-75005, France
7 Brain Physiology Laboratory, Université Paris Descartes, PRES Sorbonne Paris Cité, Paris F-75006, France
8 CNRS UMR 8118, Paris F-75006, France
9 Inserm U836, Grenoble Neuroscience Institute, Research Group 3, LabEx Ion Channel Science and Therapeutics, BP170, 38042 Grenoble Cedex 09, France
10 Université Joseph Fourier, Grenoble, France
Abstract: Semiconductor nanocrystals (NCs) or quantum dots (Qdots) are strong, point-like emitters that are increasingly used for biological and medical imaging. Here, we report on FRET-based Ca2+ and H+ nanobiosensors that are used for the intracellular detection of local cationic transients. For this purpose, we selected the commercially available CANdot®565QD as the donor and customized red-emitting rhodamine-based cationic indicators as the acceptor. The QDs were stably functionalized using the same SH/maleimide (NH2) cross-link chemistry that is utilized for all wanted reactants, which were mixed at the desired final stoichiometry; an ionic sensor and cell penetrating peptides were utilized to favor internalization. Our work validates the use of these constructs for the detection of intracellular Ca2+ and pH transients in living cells.
Keywords: Quantum dot biosensors, Nanoparticle surface chemistry, FRET-based Ca2+ and H+ probes, Red emitting indicator, Intracellular Ca2+ and H+ fluorometry, Cell penetrating peptide.
Type of Paper： Review
Tentative Title: Revealing Nucleic Acid Mutations Using FRET Probes
Authors: Nina P. L. Junager, Jacob Kongsted and Kira Astakhova
Affiliations: Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark
Abstract: Nucleic acid mutations are of tremendous importance in modern clinical work, biotechnology and in fundamental studies of nucleic acids. Therefore a rapid, cost effective, and reliable detection of mutations is a subject of extensive research.
Today, FRET probes are among the most frequently used tools for this task. However, multiple parameters must be taken into account in order to create efficient FRET probes which are sensitive to nucleic acid mutations.
In this review, we focus on the design principles of such probes and available computational methods which allow for their rational design. Applications of advanced, rationally designed FRET probes range from new insights into cancer heterogeneity at the single-cell level to gaining new knowledge on nucleic acid structures directly in living cells.