Special Issue "FRET Biosensors"


A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Biosensors".

Deadline for manuscript submissions: 30 November 2015

Special Issue Editors

Guest Editor
Prof. Dr. Niko Hildebrandt
NanoBioPhotonics, Institut d’Electronique Fondamentale, Université Paris-Sud 91405 Orsay Cedex, France
Website: http://www.nanofret.com
E-Mail: niko.hildebrandt@u-psud.fr
Interests: FRET; spectroscopy; imaging; diagnostics; immunoassays; multiplexing; biosensing; lanthanides; quantum dots; time-gating
Guest Editor
Dr. Igor Medintz
Laboratory for Biosensors and Biomaterials, Center for Biomolecular Science & Engineering, United States Naval Research Laboratory, Washington, D.C. USA
Website: http://degruyteropen.com/people/imedintz/
E-Mail: Igor.medintz@nrl.navy.mil
Fax: +1 202 7679594
Interests: nanoparticle-biological interface, energy transfer, FRET, biosensing, enzymatic catalysis at a nanoparticle interface, nanoparticle-based cellular imaging
Guest Editor
Prof. Dr. Russ Algar
Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, BC Canada V6T 1Z1, Canada
Website: https://www.chem.ubc.ca/russ-algar
E-Mail: algar@chem.ubc.ca
Phone: 604-822-2464
Interests: fluorescence; resonance energy transfer; assays, imaging; biosensing; point-of-care diagnostics; nanoparticles; enzymes; nucleic acids; surface chemistry

Special Issue Information

Dear Colleagues,

FRET or Förster resonance energy transfer is a versatile and sensitive tool for qualitative and quantitative analysis of biological interactions and processes. The access to a wide range of fluorescent materials, in conjunction with improved, easy-to-use, and yet very sophisticated microscopes and spectrometers, have made FRET a very prominent technique for biosensing. Fluorophores that are utilized in FRET now encompass organic dyes, fluorescent proteins, semiconductor quantum dots, metal chelates, various noble metal and other nanoparticles, intrinsically fluorescent amino acids, biological cofactors, and polymers, to name but a few members of this growing library. Hand-in-hand with materials development is the growing availability of numerous reactive and bioorthogonal chemistries to specifically attach such fluorophores to all types of biological molecules, ranging from proteins to DNA. The unique ability of FRET to probe nanoscale inter- and intramolecular separation distances, has also led to a rapidly growing field of structural FRET studies of biomolecules and biological complexes.

We invite manuscripts for this forthcoming Special Issue that describe all aspects pertinent to FRET-based biosensing and bioimaging. Both reviews and original research articles will be published. Reviews should provide an up-to-date and critical overview of the current state of the art in a particular application, such as diagnostics and protein–protein interactions, or a particular technique such as single-molecule FRET or FRET spectroscopic rulers. Original research papers that describe the utilization of FRET in biosensing, or new concepts and fundamental studies with potential relevance to biosensing, are also of interest. If you have a preliminary idea or suggestion you would like to discuss beforehand, please feel free to contact us. We look forward to and welcome your participation in this Special Issue.

Prof. Dr. Niko Hildebrandt
Dr. Igor Medintz
Prof. Dr. Russ Algar
Guest Editors


Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. Papers will be published continuously (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are refereed through a peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Sensors is an international peer-reviewed Open Access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs).


  • FRET
  • fluorescence
  • luminescence
  • nanotechnology
  • nanomaterials
  • fluorescent probes
  • spectroscopy
  • imaging
  • bioanalysis
  • diagnostics

Published Papers (4 papers)

Download All Papers
Sort by:
Display options:
Select articles Export citation of selected articles as:
Select/unselect all
Displaying article 1-4
p. 11076-11091
by , , , ,  and
Sensors 2015, 15(5), 11076-11091; doi:10.3390/s150511076
Received: 2 April 2015 / Revised: 1 May 2015 / Accepted: 6 May 2015 / Published: 12 May 2015
Show/Hide Abstract | PDF Full-text (4972 KB) | Supplementary Files
(This article belongs to the Special Issue FRET Biosensors)
abstract graphic
p. 9265-9276
by  and
Sensors 2015, 15(4), 9265-9276; doi:10.3390/s150409265
Received: 27 February 2015 / Revised: 3 April 2015 / Accepted: 14 April 2015 / Published: 21 April 2015
Show/Hide Abstract | PDF Full-text (940 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue FRET Biosensors)
abstract graphic
p. 8852-8865
by  and
Sensors 2015, 15(4), 8852-8865; doi:10.3390/s150408852
Received: 22 January 2015 / Revised: 2 April 2015 / Accepted: 9 April 2015 / Published: 15 April 2015
Show/Hide Abstract | PDF Full-text (1733 KB) | HTML Full-text | XML Full-text | Supplementary Files
(This article belongs to the Special Issue FRET Biosensors)
p. 14488-14499
by , , , , ,  and
Sensors 2014, 14(8), 14488-14499; doi:10.3390/s140814488
Received: 25 June 2014 / Revised: 1 August 2014 / Accepted: 5 August 2014 / Published: 8 August 2014
Show/Hide Abstract | PDF Full-text (1373 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue FRET Biosensors)
Select/unselect all
Displaying article 1-4
Select articles Export citation of selected articles as:

Planned Papers

The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.

Type of Paper: Article
Title: Optimization of a red-shifted FRET based cameleon within the endoplasmic reticulum
Authors: Markus Waldeck-Weiermair*, Helmut Bischof, Sandra Blass, Andras T. Deak, Clara Roller, Christiane Klec, Thomas Graier, Rene Rost, Nicole A. Hofmann, Wolfgang F. Graier, Roland Malli
Affiliation: Institute of Molecular Biology and Biochemistry, Research Unit for Molecular and Cellular Physiology, Center of Molecular Medicine, Medical University of Graz, Harrachgasse 21/III, Graz, Austria; Phone: +43-316-380-7562; Fax: +43-316-380-9615; E-mail: markus.waldeckweiermair@medunigraz.at
The endoplasmic reticulum (ER) represents the intracellular calcium (Ca2+) store. Until now, the FRET-based ER targeted Ca2+ sensor (called D1ER) was used to determine the ER Ca2+ concentration ([Ca2+]ER) within single cells. However, because of overlaps between excitation wavelengths, this probe is unsuitable for simultaneous recording when such recordings are performed with [Ca2+]cyto using Fura2. Based on our recently developed, mitochondria-targeted, red shifted FRET sensor, which monitors both [Ca2+]mito and [Ca2+]cyto, we have constructed an ER-targeted version of this sensor. However, this probe lost its sensitivity to Ca2+ within the ER. Hence, we herein describe the optimization of an ER targeted, FRET based sensor. By replacing the green donor and/or red/orange acceptor proteins with various other fluorescence proteins, and considering their orientation within the sensor molecule, we have developed multiple indicators that help the sensor regain its sensitivity to Ca2+ when targeted to the ER. Thus, we have obtained an optimized red-shifted chameleon that simultaneously senses, in a ratiometric manner, both [Ca2+]ER and [Ca2+]cyto within same individual cells.

Title: Towards cellular imaging of Ca2+ and H+ microdomains
Alsu I. Zamaleeva, 1,2,3,*, Despras Guillaume, 4,5,6, Camilla Luccardini, 1,2,3, Mayeul Collot, 4,5,6, Martin Oheim 7,8, Michel De Waard 9,10, Jean-Maurice Mallet 4,5,6, Anne Feltz 1,2,3,*
Ecole Normale Supérieure, Institut de Biologie de l'ENS (IBENS), Paris F-75005, France
INSERM U1024, Paris F-75005, France
CNRS UMR 8197, Paris F-75005, France
UPMC Université́ Paris 06, Ecole Normale Supérieure (ENS), Paris, F-75005 France
CNRS UMR 7203, Paris F-75005, France
Laboratory of Biomolecules (LBM), Paris F-75005, France
Brain Physiology Laboratory, Université Paris Descartes, PRES Sorbonne Paris Cité, Paris F-75006, France
CNRS UMR 8118, Paris F-75006, France
Inserm U836, Grenoble Neuroscience Institute, Research Group 3, LabEx Ion Channel Science and Therapeutics, BP170, 38042 Grenoble Cedex 09, France
Université Joseph Fourier, Grenoble, France
Semiconductor nanocrystals (NCs) or quantum dots (Qdots) are strong, point-like emitters that are increasingly used for biological and medical imaging. Here, we report on FRET-based Ca2+ and H+ nanobiosensors that are used for the intracellular detection of local cationic transients. For this purpose, we selected the commercially available CANdot®565QD as the donor and customized red-emitting rhodamine-based cationic indicators as the acceptor. The QDs were stably functionalized using the same SH/maleimide (NH2) cross-link chemistry that is utilized for all wanted reactants, which were mixed at the desired final stoichiometry; an ionic sensor and cell penetrating peptides were utilized to favor internalization. Our work validates the use of these constructs for the detection of intracellular Ca2+ and pH transients in living cells.
Quantum dot biosensors, Nanoparticle surface chemistry, FRET-based Ca2+ and H+ probes, Red emitting indicator, Intracellular Ca2+ and H+ fluorometry, Cell penetrating peptide.

Last update: 31 March 2015

Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert