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Sensors 2016, 16(2), 258; doi:10.3390/s16020258

Detection of Gold Nanoparticles Aggregation Growth Induced by Nucleic Acid through Laser Scanning Confocal Microscopy

1
Physics Department, University of Calabria, Rende 87036, Italy
2
Institute of Cybernetics of the Georgian Technical University, Euli str. 5, 0175 Tbilisi, Georgia
3
CNR-Nanotec UOS di Cosenza, c/o University of Calabria, Rende 87036, Italy
*
Author to whom correspondence should be addressed.
Academic Editors: Niko Hildebrandt, Igor Medintz and Russ Algar
Received: 16 October 2015 / Revised: 17 January 2016 / Accepted: 16 February 2016 / Published: 19 February 2016
(This article belongs to the Special Issue FRET Biosensors)
View Full-Text   |   Download PDF [1264 KB, uploaded 23 February 2016]   |  

Abstract

The gold nanoparticle (GNP) aggregation growth induced by deoxyribonucleic acid (DNA) is studied by laser scanning confocal and environmental scanning electron microscopies. As in the investigated case the direct light scattering analysis is not suitable, we observe the behavior of the fluorescence produced by a dye and we detect the aggregation by the shift and the broadening of the fluorescence peak. Results of laser scanning confocal microscopy images and the fluorescence emission spectra from lambda scan mode suggest, in fact, that the intruding of the hydrophobic moiety of the probe within the cationic surfactants bilayer film coating GNPs results in a Förster resonance energy transfer. The environmental scanning electron microscopy images show that DNA molecules act as template to assemble GNPs into three-dimensional structures which are reminiscent of the DNA helix. This study is useful to design better nanobiotechnological devices using GNPs and DNA. View Full-Text
Keywords: gold nanoparticle aggregation; deoxyribonucleic acid; laser scanning confocal microscopy; surface plasmon resonance; Förster resonance energy transfer; hydrophobicity gold nanoparticle aggregation; deoxyribonucleic acid; laser scanning confocal microscopy; surface plasmon resonance; Förster resonance energy transfer; hydrophobicity
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Gary, R.; Carbone, G.; Petriashvili, G.; De Santo, M.P.; Barberi, R. Detection of Gold Nanoparticles Aggregation Growth Induced by Nucleic Acid through Laser Scanning Confocal Microscopy. Sensors 2016, 16, 258.

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