Evaluating Quantum Dot Performance in Homogeneous FRET Immunoassays for Prostate Specific Antigen
AbstractThe integration of semiconductor quantum dots (QDs) into homogeneous Förster resonance energy transfer (FRET) immunoassay kits for clinical diagnostics can provide significant advantages concerning multiplexing and sensitivity. Here we present a facile and functional QD-antibody conjugation method using three commercially available QDs with different photoluminescence (PL) maxima (605 nm, 655 nm, and 705 nm). The QD-antibody conjugates were successfully applied for FRET immunoassays against prostate specific antigen (PSA) in 50 µL serum samples using Lumi4-Tb (Tb) antibody conjugates as FRET donors and time-gated PL detection on a KRYPTOR clinical plate reader. Förster distance and Tb donor background PL were directly related to the analytical sensitivity for PSA, ...which resulted in the lowest limits of detection for Tb-QD705 (2 ng/mL), followed by Tb-QD655 (4 ng/mL), and Tb-QD605 (23 ng/mL). Duplexed PSA detection using the Tb-QD655 and Tb-QD705 FRET-pairs demonstrated the multiplexing ability of our immunoassays. Our results show that FRET based on QD acceptors is suitable for multiplexed and sensitive biomarker detection in clinical diagnostics. View Full-Text
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Bhuckory, S.; Lefebvre, O.; Qiu, X.; Wegner, K.D.; Hildebrandt, N. Evaluating Quantum Dot Performance in Homogeneous FRET Immunoassays for Prostate Specific Antigen. Sensors 2016, 16, 197.
Bhuckory S, Lefebvre O, Qiu X, Wegner KD, Hildebrandt N. Evaluating Quantum Dot Performance in Homogeneous FRET Immunoassays for Prostate Specific Antigen. Sensors. 2016; 16(2):197.Chicago/Turabian Style
Bhuckory, Shashi; Lefebvre, Olivier; Qiu, Xue; Wegner, Karl D.; Hildebrandt, Niko. 2016. "Evaluating Quantum Dot Performance in Homogeneous FRET Immunoassays for Prostate Specific Antigen." Sensors 16, no. 2: 197.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.