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Sensors 2016, 16(8), 1173; doi:10.3390/s16081173

Revealing Nucleic Acid Mutations Using Förster Resonance Energy Transfer-Based Probes

Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark
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Author to whom correspondence should be addressed.
Academic Editors: Niko Hildebrandt, Igor Medintz and Russ Algar
Received: 19 June 2016 / Revised: 18 July 2016 / Accepted: 19 July 2016 / Published: 27 July 2016
(This article belongs to the Special Issue FRET Biosensors)
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Abstract

Nucleic acid mutations are of tremendous importance in modern clinical work, biotechnology and in fundamental studies of nucleic acids. Therefore, rapid, cost-effective and reliable detection of mutations is an object of extensive research. Today, Förster resonance energy transfer (FRET) probes are among the most often used tools for the detection of nucleic acids and in particular, for the detection of mutations. However, multiple parameters must be taken into account in order to create efficient FRET probes that are sensitive to nucleic acid mutations. In this review; we focus on the design principles for such probes and available computational methods that allow for their rational design. Applications of advanced, rationally designed FRET probes range from new insights into cellular heterogeneity to gaining new knowledge of nucleic acid structures directly in living cells. View Full-Text
Keywords: nucleic acid; mutation; fluorescence; FRET; molecular beacon; binary probe; computational strategies; in vitro hybridization nucleic acid; mutation; fluorescence; FRET; molecular beacon; binary probe; computational strategies; in vitro hybridization
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Junager, N.P.L.; Kongsted, J.; Astakhova, K. Revealing Nucleic Acid Mutations Using Förster Resonance Energy Transfer-Based Probes. Sensors 2016, 16, 1173.

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