Anchoring of FRET Sensors—A Requirement for Spatiotemporal Resolution
AbstractFRET biosensors have become a routine tool for investigating mechanisms and components of cell signaling. Strategies for improving them for particular applications are continuously sought. One important aspect to consider when designing FRET probes is the dynamic distribution and propagation of signals within living cells. We have addressed this issue by directly comparing an anchored (taFS) to a non-anchored (naFS) cleavable FRET sensor. We chose a microtubule-associated protein tau as an anchor, as microtubules are abundant throughout the cytosol of cells. We show that tau-anchored FRET sensors are concentrated at the cytoskeleton and enriched in the neurite-like processes of cells, providing high intensity of the total signal. In addition, anchoring limits the diffusion of the sensor, enabling spatiotemporally resolved monitoring of subcellular variations in enzyme activity. Thus, anchoring is an important aspect to consider when designing FRET sensors for deeper understanding of cell signaling. View Full-Text
- Supplementary File 1:
ZIP-Document (ZIP, 13175 KB)
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Ivanova, E.V.; Figueroa, R.A.; Gatsinzi, T.; Hallberg, E.; Iverfeldt, K. Anchoring of FRET Sensors—A Requirement for Spatiotemporal Resolution. Sensors 2016, 16, 703.
Ivanova EV, Figueroa RA, Gatsinzi T, Hallberg E, Iverfeldt K. Anchoring of FRET Sensors—A Requirement for Spatiotemporal Resolution. Sensors. 2016; 16(5):703.Chicago/Turabian Style
Ivanova, Elena V.; Figueroa, Ricardo A.; Gatsinzi, Tom; Hallberg, Einar; Iverfeldt, Kerstin. 2016. "Anchoring of FRET Sensors—A Requirement for Spatiotemporal Resolution." Sensors 16, no. 5: 703.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.