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Sensors 2015, 15(8), 17977-17989; doi:10.3390/s150817977

Zn(II)-Coordinated Quantum Dot-FRET Nanosensors for the Detection of Protein Kinase Activity

1
Department of Life Science, Hanyang University, Seoul 133-791, Korea
2
Research Institute for Natural Sciences, Hanyang University, Seoul 133-791, Korea
3
Department of Convergence Medicine, Asan Institute for Life Sciences, University of Ulsan College of Medicine, Asan Medical Center, Seoul 138-736, Korea
4
Research Institute for Convergence of Basic Sciences, Hanyang University, Seoul 133-791, Korea
*
Authors to whom correspondence should be addressed.
Academic Editor: Niko Hildebrandt
Received: 4 June 2015 / Revised: 18 July 2015 / Accepted: 21 July 2015 / Published: 23 July 2015
(This article belongs to the Special Issue FRET Biosensors)
View Full-Text   |   Download PDF [4592 KB, uploaded 23 July 2015]   |  

Abstract

We report a simple detection of protein kinase activity using Zn(II)-mediated fluorescent resonance energy transfer (FRET) between quantum dots (QDs) and dye-tethered peptides. With neither complex chemical ligands nor surface modification of QDs, Zn(II) was the only metal ion that enabled the phosphorylated peptides to be strongly attached on the carboxyl groups of the QD surface via metal coordination, thus leading to a significant FRET efficiency. As a result, protein kinase activity in intermixed solution was efficiently detected by QD-FRET via Zn(II) coordination, especially when the peptide substrate was combined with affinity-based purification. We also found that mono- and di-phosphorylation in the peptide substrate could be discriminated by the Zn(II)-mediated QD-FRET. Our approach is expected to find applications for studying physiological function and signal transduction with respect to protein kinase activity. View Full-Text
Keywords: FRET; quantum dot; zinc; kinase; phosphorylation FRET; quantum dot; zinc; kinase; phosphorylation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lim, B.; Park, J.-I.; Lee, K.J.; Lee, J.-W.; Kim, T.-W.; Kim, Y.-P. Zn(II)-Coordinated Quantum Dot-FRET Nanosensors for the Detection of Protein Kinase Activity. Sensors 2015, 15, 17977-17989.

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