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Sensors 2014, 14(8), 14488-14499; https://doi.org/10.3390/s140814488

Genotyping Single Nucleotide Polymorphisms Using Different Molecular Beacon Multiplexed within a Suspended Core Optical Fiber

1
Institute for Photonics and Advanced Sensing (IPAS) and The ARC Centre for Nanoscale Biophotonics, The University of Adelaide, Adelaide 5005, Australia
2
Information Engineering Department, University of Parma, Parma 43100, Italy
3
Australian Centre for Ancient DNA (ACAD), The University of Adelaide, Adelaide 5005, Australia
*
Author to whom correspondence should be addressed.
Received: 25 June 2014 / Revised: 1 August 2014 / Accepted: 5 August 2014 / Published: 8 August 2014
(This article belongs to the Special Issue FRET Biosensors)
View Full-Text   |   Download PDF [1373 KB, uploaded 8 August 2014]

Abstract

We report a novel approach to genotyping single nucleotide polymorphisms (SNPs) using molecular beacons in conjunction with a suspended core optical fiber (SCF). Target DNA sequences corresponding to the wild- or mutant-type have been accurately recognized by immobilizing two different molecular beacons on the core of a SCF. The two molecular beacons differ by one base in the loop-probe and utilize different fluorescent indicators. Single-color fluorescence enhancement was obtained when the immobilized SCFs were filled with a solution containing either wild-type or mutant-type sequence (homozygous sample), while filling the immobilized SCF with solution containing both wild- and mutant-type sequences resulted in dual-color fluorescence enhancement, indicating a heterozygous sample. The genotyping was realized amplification-free and with ultra low-volume for the required DNA solution (nano-liter). This is, to our knowledge, the first genotyping device based on the combination of optical fiber and molecular beacons. View Full-Text
Keywords: genotyping; suspended core optical fiber; molecular beacon; multiplexing; DNA detection genotyping; suspended core optical fiber; molecular beacon; multiplexing; DNA detection
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Nguyen, L.V.; Giannetti, S.; Warren-Smith, S.; Cooper, A.; Selleri, S.; Cucinotta, A.; Monro, T. Genotyping Single Nucleotide Polymorphisms Using Different Molecular Beacon Multiplexed within a Suspended Core Optical Fiber. Sensors 2014, 14, 14488-14499.

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