Special Issue "Amperometric Biosensors"


A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Biosensors".

Deadline for manuscript submissions: 31 October 2014

Special Issue Editor

Guest Editor
Dr. Roberto Pilloton
1st Researcher at CNR Institute for Atmospheric Pollution CNR - Via Salaria km 29, 300, Monterotondo, Rome, Italy
Website: http://www.biosensing.net
E-Mail: pilloton@iia.cnr.it
Phone: +39 06 49935282
Fax: +39 06 41522270
Interests: electrochemistry, environmental analytical chemistry; biosensors; sensors and sensing; continuous flow monitoring; immobilization techniques; enzyme inhibitors; lab on a Chip; nanostructured electrodes; screen printed electrodes; herbicides; pesticides; phenolic compounds; cholinesterases; photosystem II; laccase; tyrosinase; immobilized cells

Special Issue Information

Dear Colleagues,

More than 50 years ago, Clark and Lyons created the first biosensor: a glucose amperometric sensor. They coupled a Clark amperometric sensor for dissolved oxygen with an enzyme, glucose oxidase, which was immobilized on the tip of the electrode with a cellophane membrane. This method of bio-quantitative-assaying was simple, quick, and cheap. In subsequent pioneering years, the assay was extensively and profitably developed for diabetes monitoring.

Since that time, many improvements and applications were developed to not only increase the analytical performance of these amperometric biosensors, but also to prolong the lifetime of several immobilized biological molecules. Developments concerned such molecules’ oriented immobilization, the miniaturization of transducers, and automated, long-term monitoring applications with respect to several fields, which range from medicine, to environmental science and food quality studies.

Currently, improvements in amperometric biosensors mainly concern new materials for disposable electrodes, their deposition techniques (i.e., screen and ink-jet printing) and nanostructures (i.e., nanoelectrode ensembles), engineered sensing biological molecules, their electrochemical addressing and reversible and oriented immobilization, micro-fluidic devices, and Lab-on-a-Chip devices. These improvements reflect the cross interaction of several disciplines and technologies, which range from (without being exhaustive) chemistry, biology, physics, and molecular biology, to nanotechnology, micro-fabrication, and electronic engineering.

This Special Issue aims to bring together articles discussing innovative applications of amperometric biosensors, and to share the benefit of these new ideas and concepts, which are employed in multiple fields, with authors and readers of the journal, who have varying interests.

Both review articles and original research papers relating to the application of amperometric biosensors are solicited.

Dr. Roberto Pilloton
Guest Editor


Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. Papers will be published continuously (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are refereed through a peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Sensors is an international peer-reviewed Open Access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs).


  • amperometric biosensors
  • nanostructured particles and electrodes
  • carbon nanotubes and graphene
  • engineered molecules or microorganisms
  • continuous flow monitoring
  • immobilization techniques
  • lab on a chip
  • screen printed electrode

Published Papers (6 papers)

by , , ,  and
Sensors 2014, 14(6), 11097-11109; doi:10.3390/s140611097
Received: 15 May 2014; in revised form: 12 June 2014 / Accepted: 17 June 2014 / Published: 23 June 2014
Show/Hide Abstract | PDF Full-text (645 KB) | HTML Full-text | XML Full-text

by , ,  and
Sensors 2014, 14(5), 8203-8216; doi:10.3390/s140508203
Received: 7 March 2014; in revised form: 23 April 2014 / Accepted: 28 April 2014 / Published: 7 May 2014
Show/Hide Abstract | PDF Full-text (690 KB) | HTML Full-text | XML Full-text

by , ,  and
Sensors 2014, 14(3), 4634-4656; doi:10.3390/s140304634
Received: 17 December 2013; in revised form: 28 February 2014 / Accepted: 5 March 2014 / Published: 7 March 2014
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by , ,  and
Sensors 2014, 14(2), 3756-3767; doi:10.3390/s140203756
Received: 10 December 2013; in revised form: 24 January 2014 / Accepted: 8 February 2014 / Published: 24 February 2014
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abstract graphic

by , , , ,  and
Sensors 2014, 14(2), 3543-3556; doi:10.3390/s140203543
Received: 29 November 2013; in revised form: 28 January 2014 / Accepted: 12 February 2014 / Published: 20 February 2014
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by , ,  and
Sensors 2014, 14(2), 2578-2594; doi:10.3390/s140202578
Received: 10 December 2013; in revised form: 26 January 2014 / Accepted: 27 January 2014 / Published: 7 February 2014
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abstract graphic

Planned Papers

The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.

Type of Paper: Article
Donatella Albanese, Francesca Malvano, Adriana Sannini, Marisa Di Matteo
Department of Industrial Engineering, University of Salerno, Via Giovanni Paolo II ,132, 84084 Fisciano (SA) Italy
Gluconic acid is one of the most important metabolite associated with grape infection by Botrytis cinerea. This pathogen occurs worldwide and gives problems during alcoholic fermentations, post-fermentation and wine storage. The use of NADPH dehydrogenase enzymes for gluconic acid determination by biosensor requires high overpotential (ca. 1V vs SCE) for the direct electrochemical oxidation of NAD(P)H which is accompanied by electrode fouling. This study reports the developing of screen printed amperometric biosensor for the determination of gluconic acid based on the ienzymatic system, gluconokinase plus 6-phospho-D-gluconate dehydrogenase, coimmobilized into polyaniline-poly(2-acrylamido-2-methyl-1-propane)(PANI-PAAMPSA) polymer. Optimization steps in terms of electroactivity of PANI-PAAMPSA film, analytical biosensor performances and measurements of gluconate content in real grape samples have been performed.

Type of Paper: Article
Title: An Amperometric Biosensor Method for Checking the Antioxidant Capacity of Several Wood Fruits: Comparing Spectrophotometric and Fluorimetric Methods
Author: Mauro Tomassetti
Affiliation: Department of Chemistry, “Sapienza” University of Rome, p.le A. Moro,5, Rome, 00185, Italy. E-Mail: mauro.tomassetti@uniroma1.it
Abstract: Wood fruit represents protective factors of fundamental importance for human health, due to their high content of antioxidant compounds. Therefore, in the case of physical deficiencies, or when food intake is insufficient for providing enough antioxidant nutrients, the consumption of different types of fruit, and in particular, of wood fruit, is recommended. This has become a widespread practice, although the antioxidant properties of such fruit are often not fully quantified. It is thus of particular and topical interest to be able to come up with new analytical methods of assessing the antioxidant capacity of several common varieties of wood fruit that are readily available from the greengrocer. The present work aimed to investigate the antioxidant capacity of several of these fresh wood fruit varieties, and also of yogurts, fruit juices, and jams containing them. The results of the investigation are compared and the fruit varieties and products containing them ranked in the order of their antioxidant capacity. Blackberries, blueberries, currants, raspberries, and wild strawberries are rich in active principles, i.e., antioxidant compounds like polyphenols, carotenoids, anthocyanins, and ascorbic acid. Therefore, their antiradical action is of fundamental importance; in order to measure their antioxidant capacity, in addition to the various methods described in the literature, our laboratory in recent years has developed a special electrochemical method based on a superoxide dismutase (SOD) biosensor [1] to determine antioxidant capacity. The results, obtained by applying the SOD biosensor method to various fresh wood fruit varieties or wood fruit-based products, were compared with the results obtained with the spectrophotometric method based on N,N–dimethyl-p-phenylenediamine (DMPD-FeCl3). The same results were additionally compared with those obtained using the ORAC fluorimetric method, which is the most frequently used method of determining antioxidant activity in food matrices (and which was adopted as a reference method). The correlation among the three methods was satisfactory.

Type of Paper: Article
Title: Carbon Fiber Ultramicroelectrode Electrodeposited with Over-Oxidized Polypyrrole Nanocomposites for Amperometric Detection of Vesicular Exocytosis from Single Pheochromocytoma Cell
Authors: Li Wang1, 2, Huiren Xu1, 2, Jinping Luo1, Chunxiu Liu1, Juntao Liu1, Shengwei Xu1, Xinxia Cai1,2, *
Affiliation: 1 State Key Laboratory of Transducer Technology, Institute of Electronics Chinese Academy of Sciences, Beijing 100190, China; E-mail: xxcai@mail.ie.ac.cn Tel: +86-10-58887193
Fax: +86-10-58887172.
University of Chinese Academy of Sciences, Beijing 100190, China
Abstract: Vesicular exocytosis is ubiquitous, but is difficult to detect within the cells’ communication mechanism. A 6 µm ultramicrodic carbon fiber electrode electrodeposited with over-oxidized polypyrrole nanocomposites (PPyox-CFE) was fabricated, which was applied successfully for real-time monitoring quantal exocytosis from individual pheochromocytoma (PC12) cells. PPyox-CFE was evaluated by ferricyanide ion and dopamine (DA) solutions through cyclic voltammetry and amperometry electrochemical methods, and results revealed that PPyox-CFE improved the detection limit of DA and increased reversibility of ferricyanide ion. In particular, the sensitivity of DA was improved to 1486.2 nA µM -1 µm-2 using the PPyox-CFE combination. The ultramicrodic electrode combined with the patch-clamp system, for which the potential and sampling rate were maintained at 650 mV and 10 kHz respectively, was used to detect vesicular exocytosis of DA from individual PC12 cells with 100 mM K+ stimulation. A total of 193 spikes were obtained with a mean duration of 6 ms, the amplitude was 110 pA and signal-to-noise ratio was 5.5, during 25 s amperometry recording. PPyox-CFE, with its capability of detecting vesicular exocytosis, has potential application in neurons’ communication research.
Keywords: amperometry; ultramicrodic carbon fiber electrode;over-oxidized polypyrrole nanocomposites;vesicular exocytosis

Type of Paper: Article
Selective Recognition of 5-Hydroxytryptamine and Dopamine on Multi-Walled Carbon Nanotubes-Chitosan Hybrid Film Modified Microelectrode Array
Huiren Xu 1, 2, Li Wang 1, 2, Jinping Luo1, Chunxiu Liu1, Juntao Liu1, Song Zhang 1, 2, Xinxia Cai1,2, *
State Key Laboratory of Transducer Technology, Institute of Electronics Chinese Academy of Sciences, Beijing 100190, China; E-mail: xxcai@mail.ie.ac.cn Tel: +86-10-58887193  Fax: +86-10-58887172
University of Chinese Academy of Sciences, Beijing 100190, China
Abstract: It is difficult to determine dopamine (DA) and 5-hydroxytryptamine (5-HT) accurately because of the interference of ascorbic acid (AA) in vitro, which has a high concentration and can be oxidized potentially close to that of DA and 5-HT at bare electrode, combined with the overlapping voltammetric signal of DA and 5-HT at bare electrode. Herein, chitosan (CS) was used as a stabilizing matrix by electrochemical reaction which changed chitosan from a close to working electrode soluble into being locally insoluble by removal of local H+. Results revealed that the hybrid film showing excellent catalytic activity can resolve the overlapping anodic peaks of DA and 5-HT into two well-defined peaks with separation reaching 170mV and, further, negatively shifts the oxidation peak potentials of DA and 5-HT which were 103mV and 273mV respectively, while the modified electrode showed higher cyclic voltammetry current response than the bare Au electrode. Therefore, this work was used for selective molecular recognition of DA and 5-HT using the chronoamperometry at different working potential which has potential application for real-time detection in in vitro experiments.
Keywords: chitosan;microelectrode array;multi-walled nanotubes; dopamine;5-hydroxytryptamine

Last update: 17 June 2014

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