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Sensors 2014, 14(5), 8203-8216; doi:10.3390/s140508203

Acetylcholinesterase Inhibition-Based Biosensor for Aluminum(III) Chronoamperometric Determination in Aqueous Media

1
University of Costa Rica, CELEQ, San Pedro de Montes de Oca, 11500-2060 San José, Costa Rica
2
Department of Chemistry, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos, Spain
*
Author to whom correspondence should be addressed.
Received: 7 March 2014 / Revised: 23 April 2014 / Accepted: 28 April 2014 / Published: 7 May 2014
(This article belongs to the Special Issue Amperometric Biosensors)
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Abstract

A novel amperometric biosensor for the determination of Al(III) based on the inhibition of the enzyme acetylcholinesterase has been developed. The immobilization of the enzyme was performed on screen-printed carbon electrodes modified with gold nanoparticles. The oxidation signal of acetylthiocholine iodide enzyme substrate was affected by the presence of Al(III) ions leading to a decrease in the amperometric current. The developed system has a detection limit of 2.1 ± 0.1 μM for Al(III). The reproducibility of the method is 8.1% (n = 4). Main interferences include Mo(VI), W(VI) and Hg(II) ions. The developed method was successfully applied to the determination of Al(III) in spiked tap water . The analysis of a certified standard reference material was also carried out. Both results agree with the certified values considering the respective associated uncertainties. View Full-Text
Keywords: acetylcholinesterase; biosensor; aluminum; acetylthiocholine iodide; screen-printed electrodes; gold nanoparticles acetylcholinesterase; biosensor; aluminum; acetylthiocholine iodide; screen-printed electrodes; gold nanoparticles
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Barquero-Quirós, M.; Domínguez-Renedo, O.; Alonso-Lomillo, M.A.; Arcos-Martínez, M.J. Acetylcholinesterase Inhibition-Based Biosensor for Aluminum(III) Chronoamperometric Determination in Aqueous Media. Sensors 2014, 14, 8203-8216.

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