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Sensors 2014, 14(5), 8203-8216; doi:10.3390/s140508203
Article

Acetylcholinesterase Inhibition-Based Biosensor for Aluminum(III) Chronoamperometric Determination in Aqueous Media

1,* , 2
, 2
 and 2
Received: 7 March 2014; in revised form: 23 April 2014 / Accepted: 28 April 2014 / Published: 7 May 2014
(This article belongs to the Special Issue Amperometric Biosensors)
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Abstract: A novel amperometric biosensor for the determination of Al(III) based on the inhibition of the enzyme acetylcholinesterase has been developed. The immobilization of the enzyme was performed on screen-printed carbon electrodes modified with gold nanoparticles. The oxidation signal of acetylthiocholine iodide enzyme substrate was affected by the presence of Al(III) ions leading to a decrease in the amperometric current. The developed system has a detection limit of 2.1 ± 0.1 μM for Al(III). The reproducibility of the method is 8.1% (n = 4). Main interferences include Mo(VI), W(VI) and Hg(II) ions. The developed method was successfully applied to the determination of Al(III) in spiked tap water . The analysis of a certified standard reference material was also carried out. Both results agree with the certified values considering the respective associated uncertainties.
Keywords: acetylcholinesterase; biosensor; aluminum; acetylthiocholine iodide; screen-printed electrodes; gold nanoparticles acetylcholinesterase; biosensor; aluminum; acetylthiocholine iodide; screen-printed electrodes; gold nanoparticles
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Barquero-Quirós, M.; Domínguez-Renedo, O.; Alonso-Lomillo, M.A.; Arcos-Martínez, M.J. Acetylcholinesterase Inhibition-Based Biosensor for Aluminum(III) Chronoamperometric Determination in Aqueous Media. Sensors 2014, 14, 8203-8216.

AMA Style

Barquero-Quirós M, Domínguez-Renedo O, Alonso-Lomillo MA, Arcos-Martínez MJ. Acetylcholinesterase Inhibition-Based Biosensor for Aluminum(III) Chronoamperometric Determination in Aqueous Media. Sensors. 2014; 14(5):8203-8216.

Chicago/Turabian Style

Barquero-Quirós, Miriam; Domínguez-Renedo, Olga; Alonso-Lomillo, Maria A.; Arcos-Martínez, María J. 2014. "Acetylcholinesterase Inhibition-Based Biosensor for Aluminum(III) Chronoamperometric Determination in Aqueous Media." Sensors 14, no. 5: 8203-8216.


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