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Sensors 2014, 14(2), 3756-3767; doi:10.3390/s140203756
Article

A Disposable Alkaline Phosphatase-Based Biosensor for Vanadium Chronoamperometric Determination

1,* , 2
, 2
 and 2
1 CELEQ and School of Chemistry, University of Costa Rica, San Pedro de Montes de Oca, San José P.O. Box 11500-2060, Costa Rica 2 Department of Chemistry, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos s/n, Burgos 09001, Spain
* Author to whom correspondence should be addressed.
Received: 10 December 2013 / Revised: 24 January 2014 / Accepted: 8 February 2014 / Published: 24 February 2014
(This article belongs to the Special Issue Amperometric Biosensors)
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Abstract

A chronoamperometric method for vanadium ion determination, based on the inhibition of the enzyme alkaline phosphatase, is reported. Screen-printed carbon electrodes modified with gold nanoparticles were used as transducers for the immobilization of the enzyme. The enzymatic activity over 4-nitrophenyl phosphate sodium salt is affected by vanadium ions, which results in a decrease in the chronoamperometric current registered. The developed method has a detection limit of 0.39 ± 0.06 µM, a repeatability of 7.7% (n = 4) and a reproducibility of 8% (n = 3). A study of the possible interferences shows that the presence of Mo(VI), Cr(III), Ca(II) and W(VI), may affect vanadium determination at concentration higher than 1.0 mM. The method was successfully applied to the determination of vanadium in spiked tap water.
Keywords: alkaline phosphatase; biosensor; gold nanoparticles; 4-nitrophenyl phosphate; screen printed electrode; vanadium; water analysis alkaline phosphatase; biosensor; gold nanoparticles; 4-nitrophenyl phosphate; screen printed electrode; vanadium; water analysis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Alvarado-Gámez, A.L.; Alonso-Lomillo, M.A.; Domínguez-Renedo, O.; Arcos-Martínez, M.J. A Disposable Alkaline Phosphatase-Based Biosensor for Vanadium Chronoamperometric Determination. Sensors 2014, 14, 3756-3767.

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