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Sensors 2014, 14(2), 3543-3556; doi:10.3390/s140203543
Article

Laccase Biosensor Based on Electrospun Copper/Carbon Composite Nanofibers for Catechol Detection

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Received: 29 November 2013; in revised form: 28 January 2014 / Accepted: 12 February 2014 / Published: 20 February 2014
(This article belongs to the Special Issue Amperometric Biosensors)
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Abstract: The study compared the biosensing properties of laccase biosensors based on carbon nanofibers (CNFs) and copper/carbon composite nanofibers (Cu/CNFs). The two kinds of nanofibers were prepared by electrospinning and carbonization under the same conditions. Scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy were employed to investigate the morphologies and structures of CNFs and Cu/CNFs. The amperometric results indicated that the Cu/CNFs/laccase(Lac)/Nafion/glass carbon electrode (GCE) possessed reliable analytical performance for the detection of catechol. The sensitivity of the Cu/CNFs/Lac/Nafion/GCE reached 33.1 μA/mM, larger than that of CNFs/Lac/Nafion/GCE. Meanwhile, Cu/CNFs/Lac/Nafion/GCE had a wider linear range from 9.95 × 10−6 to 9.76 × 10−3 M and a lower detection limit of 1.18 μM than CNFs/Lac/Nafion/GCE. Moreover, it exhibited a good repeatability, reproducibility, selectivity and long-term stability, revealing that electrospun Cu/CNFs have great potential in biosensing.
Keywords: electrospinning; copper/carbon composite nanofibers; laccase biosensor; catechol detection; electrochemical properties electrospinning; copper/carbon composite nanofibers; laccase biosensor; catechol detection; electrochemical properties
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Fu, J.; Qiao, H.; Li, D.; Luo, L.; Chen, K.; Wei, Q. Laccase Biosensor Based on Electrospun Copper/Carbon Composite Nanofibers for Catechol Detection. Sensors 2014, 14, 3543-3556.

AMA Style

Fu J, Qiao H, Li D, Luo L, Chen K, Wei Q. Laccase Biosensor Based on Electrospun Copper/Carbon Composite Nanofibers for Catechol Detection. Sensors. 2014; 14(2):3543-3556.

Chicago/Turabian Style

Fu, Jiapeng; Qiao, Hui; Li, Dawei; Luo, Lei; Chen, Ke; Wei, Qufu. 2014. "Laccase Biosensor Based on Electrospun Copper/Carbon Composite Nanofibers for Catechol Detection." Sensors 14, no. 2: 3543-3556.



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