Abstract: The study compared the biosensing properties of laccase biosensors based on carbon nanofibers (CNFs) and copper/carbon composite nanofibers (Cu/CNFs). The two kinds of nanofibers were prepared by electrospinning and carbonization under the same conditions. Scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy were employed to investigate the morphologies and structures of CNFs and Cu/CNFs. The amperometric results indicated that the Cu/CNFs/laccase(Lac)/Nafion/glass carbon electrode (GCE) possessed reliable analytical performance for the detection of catechol. The sensitivity of the Cu/CNFs/Lac/Nafion/GCE reached 33.1 μA/mM, larger than that of CNFs/Lac/Nafion/GCE. Meanwhile, Cu/CNFs/Lac/Nafion/GCE had a wider linear range from 9.95 × 10−6 to 9.76 × 10−3 M and a lower detection limit of 1.18 μM than CNFs/Lac/Nafion/GCE. Moreover, it exhibited a good repeatability, reproducibility, selectivity and long-term stability, revealing that electrospun Cu/CNFs have great potential in biosensing.
Keywords: electrospinning; copper/carbon composite nanofibers; laccase biosensor; catechol detection; electrochemical properties
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Fu, J.; Qiao, H.; Li, D.; Luo, L.; Chen, K.; Wei, Q. Laccase Biosensor Based on Electrospun Copper/Carbon Composite Nanofibers for Catechol Detection. Sensors 2014, 14, 3543-3556.
Fu J, Qiao H, Li D, Luo L, Chen K, Wei Q. Laccase Biosensor Based on Electrospun Copper/Carbon Composite Nanofibers for Catechol Detection. Sensors. 2014; 14(2):3543-3556.
Fu, Jiapeng; Qiao, Hui; Li, Dawei; Luo, Lei; Chen, Ke; Wei, Qufu. 2014. "Laccase Biosensor Based on Electrospun Copper/Carbon Composite Nanofibers for Catechol Detection." Sensors 14, no. 2: 3543-3556.