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Computational Modeling of Mediator Oxidation by Oxygen in an Amperometric Glucose Biosensor
Faculty of Mathematics and Informatics, Vilnius University, Didlaukio 47, LT-08303 Vilnius, Lithuania
Institute of Biochemistry, Vilnius University, Mokslininku 12, LT-08662 Vilnius, Lithuania
* Author to whom correspondence should be addressed.
Received: 10 December 2013; in revised form: 26 January 2014 / Accepted: 27 January 2014 / Published: 7 February 2014
Abstract: In this paper, an amperometric glucose biosensor is modeled numerically. The model is based on non-stationary reaction-diffusion type equations. The model consists of four layers. An enzyme layer lies directly on a working electrode surface. The enzyme layer is attached to an electrode by a polyvinyl alcohol (PVA) coated terylene membrane. This membrane is modeled as a PVA layer and a terylene layer, which have different diffusivities. The fourth layer of the model is the diffusion layer, which is modeled using the Nernst approach. The system of partial differential equations is solved numerically using the finite difference technique. The operation of the biosensor was analyzed computationally with special emphasis on the biosensor response sensitivity to oxygen when the experiment was carried out in aerobic conditions. Particularly, numerical experiments show that the overall biosensor response sensitivity to oxygen is insignificant. The simulation results qualitatively explain and confirm the experimentally observed biosensor behavior.
Keywords: modeling; reaction-diffusion; biosensor; oxygen; aerobic; anaerobic
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Šimelevičius, D.; Petrauskas, K.; Baronas, R.; Razumienė, J. Computational Modeling of Mediator Oxidation by Oxygen in an Amperometric Glucose Biosensor. Sensors 2014, 14, 2578-2594.
Šimelevičius D, Petrauskas K, Baronas R, Razumienė J. Computational Modeling of Mediator Oxidation by Oxygen in an Amperometric Glucose Biosensor. Sensors. 2014; 14(2):2578-2594.
Šimelevičius, Dainius; Petrauskas, Karolis; Baronas, Romas; Razumienė, Julija. 2014. "Computational Modeling of Mediator Oxidation by Oxygen in an Amperometric Glucose Biosensor." Sensors 14, no. 2: 2578-2594.