Special Issue "DNA-Based Nanotechnology"
A special issue of Nanomaterials (ISSN 2079-4991).
Deadline for manuscript submissions: closed (30 June 2016)
Assoc. Prof. Dr. Leonid Gurevich
Department of Physics and Nanotechnology, Faculty of Engineering and Science, Aalborg University, 9220 Aalborg Ø, Denmark
Unique self-assembly and recognition properties of biological molecules offer an attractive route for bottom-up construction of complex, infinitely customizable, and cost-effective nanodevices. DNA, designed by nature to store information, is arguably one of the most promising candidates for the job. From the nanotechnology prospective, DNA possess a number of attractive properties, making it a unique construction material with fantastic structuring and self-recognition properties, which also comes with a well-established enzymatic toolbox allowing to produce essentially any desired sequence with a broad range of modifications. Thus, it does not come as a surprise that a large variety of different nanostructures, including 2D and 3D DNA arrays, various DNA-nanoparticle conjugates, DNA origami, DNA robots, DNA drug-delivery system, etc., have been created during recent years.
In this Special issue, we would like to reflect the broadness of the subject and invite contributions from all walks of DNA nanotechnology, including, but not limited to, DNA origami, DNA arrays, DNA-nanoparticle conjugates, novel DNA structures, as well as applications of DNA in molecular electronics, nanophotonics, biosensing, and nanomedicine.
Assoc. Prof. Dr. Leonid Gurevich
Manuscript Submission Information
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Nanomaterials is an international peer-reviewed open access monthly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.
- DNA arrays
- DNA origami
- DNA-nanoparticle conjugates
- triplex DNA
- quadruplex DNA
- DNA nanodevices
- DNA biosensors, nanoplasmonics, nanophotonics, molecular electronics
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Title: Controlled enlargement of gold nanoparticles in DNA-nanoparticle conjugates
Authors: Gennady Eidelshtein 1, Moran Fattal 1, Gavriel Avishai 1, Clelia Giannini 2 and Alexander Kotlyar 1,*
Affiliation: 1Department of Biochemistry and Molecular Biology, George S. Wise Faculty of life Sciences and The Center of Nanoscience and Nanotechnology, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel
2 Università degli Studi di Milano, Dipartimento di Chimica, Via C. Golgi, 19, 20133 Milan, Italy
Abstract: Here, we describe a method for controlled enlargement of 5 nm gold nanoparticles attached to both sides of a double stranded DNA. The nanoparticles in these dumbbell-shaped conjugates were enlarged on mica by treatment of the surface with micromolar concentrations of gold ions and ascorbic acid. AFM imaging analysis shows that this treatment yields uniform nanoparticles which depend on the time of the treatment. By increasing the nanoparticle size, we were able to reduce the edge-to edge distance between the nanoparticles in the dumbbell-shaped conjugate from hundreds to several nanometers. These structures can serve as an experimental model for testing of electrical conductivity of DNA. The method of nanoparticles enlargement reported here can also be used for production of various DNA-nanoparticle-based molecular architectures and functional devices.
Title: Metallic nanostructures based on DNA nanoshapes
Authors: Boxuan Shen a,*, Kosti Tapio a, Mauri A. Kostiainen b, Veikko Linko b and J. Jussi Toppari a,*
a University of Jyvaskyla, Department of Physics, Nanoscience Center, P.O. Box 35, FI-40014 University of Jyväskylä, Finland.
b Biohybrid Materials, Department of Biotechnology and Chemical Technology, Aalto University, P.O. Box 16100, FI-00076 Aalto, Espoo, Finland.
E-mails: firstname.lastname@example.org, email@example.com
Abstract: Metallic nanostructures have inspired extensive research over several decades, particularly within the field of nanoelectronics and increasingly also in plasmonics. Due to the limitations of conventional lithographical methods, the development of bottom-up fabricated metallic nanostructures has become more and more in demand. In here, especially the remarkable development of DNA-based assembly has yielded many successful methods and realizations, e.g., chemical DNA metallization via seeding or ionization, as well as DNA guided molding or lithography. These methods offer higher resolution, versatility and throughput, and could enable fabrication of arbitrary nanoshapes even down to 10 nm, thus making more novel applications possible. In this review, we cover the evolution of DNA in nanometallization, starting from the metallized dsDNA for electronics all the way to plasmonic structures fabricated using DNA origamis.
Title: DNA-Based Enzyme Reactors and Systems
Authors: Veikko Linko 1,*, Sami Nummelin 1, Kosti Tapio 2, J. Jussi Toppari 2 and Mauri A. Kostiainen 1,*
Affiliation: 1 Biohybrid Materials, Department of Biotechnology and Chemical Technology, Aalto University, P.O. Box 16100, 00076 Aalto, Finland
2 University of Jyvaskyla, Department of Physics, Nanoscience Center, P.O. Box 35, 40014 University of Jyväskylä, Finland
* Correspondence: firstname.lastname@example.org (V.L); email@example.com (M.A.K.)
Abstract: During recent years, the possibility to create custom biocompatible nanoshapes using DNA as a building material has rapidly emerged. Further, these rationally designed DNA structures could be exploited in positioning pivotal molecules, such as enzymes, with nanometer-level precision. This feature could be used in fabrication of artificial biochemical machinery that are able to mimic the complex reactions found in living cells. DNA-enzyme conjugates could be used to control the (multi-enzyme) cascade reactions and to study the enzyme functions and the reaction pathways. Moreover, sophisticated DNA structures could be utilized in encapsulating active enzymes and delivering the molecular cargo into cells. In this review, we focus on the latest DNA-based enzyme systems; enzyme arrays, reactors and carriers that can find uses in various biotechnological and nanomedical applications.
Keywords: DNA nanotechnology; DNA origami; self-assembly; enzyme; cascade reactions; drug-delivery; nanomedicine
Title: From lab-scale proof-of-principle to high-volume low-cost production of lab-on-chip systems for bio-analytical applications
Author: Rafael TaboryskiTechnical University of Denmark, Department of Micro- and Nanotechnology, 2800 Lyngby, Denmark
Abstract: We provide a review of the advancement in technology for lab-on-chip (LoC) systems aimed for bio-analytical investigations. High impact applications, such as the optical mapping of single DNA-molecules, single cell ion-channel recordings, cell sorting, detection of quantal exocytosis from single cells, and on-chip polymerase chain reaction (PCR) require solutions, where chips can be disposed after use. Hence a roadmap towards a more widespread use of LoC technology among researchers, not to say a commercialization of the technology, calls for adoption of low-cost mass-production methodologies for fabrication of chips. The first LoC papers appeared more than 20 years ago and despite huge LoC research activities in the academic world, a commercialization of the technology is still sparse. We analyze the technological requirements for high volume production of LoC systems for chosen applications in respect to choice of materials, channel dimensions, and interfaces.